Transcriptome shifts in response to furfural and acetic acid in Saccharomyces cerevisiae
Transcriptome shifts in response to furfural and acetic acid in Saccharomyces cerevisiae
复制标题
酿酒酵母中糠醛和乙酸的转录组变化
DOI：
10.1007/s00253-010-2518-2
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发表时间：
2010-05-01
影响因子：
5
通讯作者：
Yuan, Ying-Jin
中科院分区：
文献类型：
--
作者：
Li, Bing-Zhi;Yuan, Ying-Jin
糠醛和乙酸是纤维素乙醇生产过程中两种常见的微生物抑制剂，但对这些抑制剂耐受的分子机制仍不清楚。在这项研究中，利用微阵列分析研究了酿酒酵母对糠醛和乙酸的全基因组转录反应。我们发现分别有 103 个和 227 个基因在对糠醛和乙酸的反应中存在差异表达。糠醛下调与转录控制和翻译控制相关的基因，同时上调应激反应基因。此外，糠醛还中断了参与必需化学物质代谢的基因转录，例如四氢叶酸、亚精胺、精胺和单磷酸核黄素。乙酸下调编码线粒体核糖体蛋白的基因和参与碳水化合物代谢和调节的基因，上调与氨基酸代谢相关的基因。结果表明，糠醛和乙酸在转录水平上对细胞代谢的多个方面都有影响，线粒体可能在糠醛和乙酸的反应中发挥重要作用。这项研究为酿酒酵母对糠醛和乙酸的分子反应提供了见解，并将有助于构建对纤维素乙醇生产更具抗性的菌株。
Furfural and acetic acid are two prevalent inhibitors to microorganisms during cellulosic ethanol production, but molecular mechanisms of tolerance to these inhibitors are still unclear. In this study, genome-wide transcriptional responses to furfural and acetic acid were investigated in Saccharomyces cerevisiae using microarray analysis. We found that 103 and 227 genes were differentially expressed in the response to furfural and acetic acid, respectively. Furfural downregulated genes related to transcriptional control and translational control, while it upregulated stress-responsive genes. Furthermore, furfural also interrupted the transcription of genes involved in metabolism of essential chemicals, such as etrahydrofolate, spermidine, spermine, and riboflavin monophosphate. Acetic acid downregulated genes encoding mitochondrial ribosomal proteins and genes involved in carbohydrate metabolism and regulation and upregulated genes related to amino acid metabolism. The results revealed that furfural and acetic acid had effects on multiple aspects of cellular metabolism on the transcriptional level and that mitochondria might play important roles in response to both furfural and acetic acid. This research has provided insights into molecular response to furfural and acetic acid in S. cerevisiae, and it will be helpful to construct more resistant strains for cellulosic ethanol production.