DISRUPTION OF ESCHERICHIA-COLI OUTER MEMBRANE-PERMEABILITY BARRIER BY IMMOBILIZED POLYMYXIN-B
DISRUPTION OF ESCHERICHIA-COLI OUTER MEMBRANE-PERMEABILITY BARRIER BY IMMOBILIZED POLYMYXIN-B
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DOI：
10.7164/antibiotics.30.1087
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发表时间：
1977-01-01
影响因子：
3.3
通讯作者：
STORM, DR
中科院分区：
文献类型：
--
作者：
ROSENTHAL, KS;STORM, DR
革兰氏阴性菌外膜系统的一个明显作用是作为选择性渗透屏障。许多对革兰氏阳性菌有活性的抗生素对革兰氏阴性菌相对无效，这可能是因为外膜隐含的屏障功能。这种解释得到了证实外膜干扰剂（如EDTA或多粘菌素B）与特定抗生素之间协同作用的研究的加强。在多粘菌素B的情况下，尚不完全清楚与其他抗菌剂的协同作用是由于外膜渗透屏障的破坏还是与内膜的相互作用。为了解决这一问题，将多粘菌素B共价连接到琼脂糖上，以限制与E.杆菌这些研究表明，固定化多粘菌素B与杆菌肽、利福平或溶菌酶协同作用。多粘菌素B表现出的协同作用可能是由于其与外膜系统的相互作用。
One of the apparent roles of the outer membrane system in gram-negative bacteria is to function as a selective permeability barrier. A number of antibiotics active against gram-positive bacteria are relatively ineffective against gram-negative bacteria presumably because of the implied barrier function of the outer membrane. This interpretation was strengthened by studies demonstrating synergism between outer membrane perturbing agents such as EDTA or polymyxin B and specific antibiotics. In the case of polymyxin B, it is not totally clear that synergism with other antimicrobials is due to disruption of the outer membrane permeability barrier or to interactions with the inner membrane. In order to resolve this question, polymyxin B was covalently attached to agarose in order to limit interactions with the outer surface of E. coli. These studies demonstrate that immobilized polymyxin B acts synergistically with bacitracin, rifampicin or lysozyme. Synergistic effects exhibited by polymyxin B may be due to its interaction with the outer membrane system.