A novel mouse gene, HemT, encoding an hematopoietic cell-specific transcript.
A novel mouse gene, HemT, encoding an hematopoietic cell-specific transcript.
复制标题
一种新的小鼠基因 HemT，编码造血细胞特异性转录本。
DOI：
10.1016/s0378-1119(99)00099-2
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发表时间：
1999
期刊：
影响因子：
3.5
通讯作者：
Bank,A
中科院分区：
文献类型：
--
作者：
Xue,H;O'Neill,D;Morrow,J;Bank,A
我们使用差异显示技术来鉴定在小鼠红白血病细胞(MELC)诱导前后差异表达的造血细胞特异性基因。在这项研究中，我们报告了一个这样的小鼠基因HEMT的鉴定、克隆和特性。通过Northern印迹、RT-PCR和原位杂交，我们发现HEMT编码三种不同的转录本。其中一种是HEMT-1，是造血细胞特异性的，可能是红系细胞特异性的。另一种是HEMT-2，主要在造血细胞中表达。HEMT-1和HEMT-2是选择性剪接产物。第三种HEMT转录本只在睾丸中表达。红系转录本在未诱导的黑色素瘤中比诱导的黑色素瘤中优先表达，并且很可能在早期红系细胞群体中表达。
We performed differential display to identify hematopoietic cell-specific genes that are expressed differentially before and after mouse erythroleukemia cell (MELC) induction. In this study we report the identification, cloning, and characterization of one such mouse gene, HemT. Using Northern blot, RT–PCR and in situ hybridization, we show that HemT encodes three different transcripts. One of them, HemT-1, is hematopoietic-cell specific and may be erythroid cell-specific. Another, HemT-2, is expressed mainly in hematopoietic cells. HemT-1 and HemT-2 are alternative splicing products. A third type of HemT transcript is expressed only in testis. The erythroid transcript is expressed preferentially in uninduced MELC as compared to induced MELC, and is likely to be expressed in early erythroid cell populations.