Ultrasensitive ELISA for the detection of hCG based on assembled gold nanoparticles induced by functional polyamidoamine dendrimers
Ultrasensitive ELISA for the detection of hCG based on assembled gold nanoparticles induced by functional polyamidoamine dendrimers
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基于功能性聚酰胺胺树枝状聚合物诱导组装的金纳米颗粒的超灵敏 ELISA 检测 hCG
DOI：
10.1016/j.aca.2018.08.038
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发表时间：
2018-12-26
影响因子：
6.2
通讯作者：
Zhang, Lei
中科院分区：
文献类型：
--
作者：
Wang, Weiguo;Li, Junmin;Zhang, Lei
放大 ELISA 信号对于疾病的早期诊断非常重要。在此，我们报道了一种基于功能性 PAMAM 诱导的 AuNP 组装的超灵敏 ELISA，用于检测 hCG。与传统 ELISA 和 mAb1-AuNP-HRP 探针相比，AuNP-PAMAM 探针具有 0.03 IU L-1 灵敏度的竞争优势。线范围为 0.1 至 6.4 IU L-1。此外，AuNP-PAMAM探针的精密度、重现性和特异性也适合hCG的检测。组装的AuNPs首先用于免疫分析中的信号增强。 (C) 2018 Elsevier B.V. 保留所有权利。
Amplifying the signal of ELISA is important in the early disease diagnosis. Herein we report an ultra-sensitive ELISA applying for the detection of hCG based on the assemble of AuNPs induced by functional PAMAM. The AuNP-PAMAM probe shows a competitive advantage sensitivity of 0.03 IU L-1 compared to traditional ELISA and mAb1-AuNP-HRP probe. The line range is ranged from 0.1 to 6.4 IU L-1. Moreover, the precision and reproducibility and specificity of AuNP-PAMAM probe are also eligible for the detection of hCG. The assembled AuNPs was firstly used in the signal enhancement in immunoassay. (C) 2018 Elsevier B.V. All rights reserved.