The effect of transient proanthocyanidins preconditioning on the cross-linking and mechanical properties of demineralized dentin
The effect of transient proanthocyanidins preconditioning on the cross-linking and mechanical properties of demineralized dentin
复制标题
瞬时原花青素预处理对脱矿牙本质交联和力学性能的影响
DOI：
10.1007/s10856-011-4430-4
复制
发表时间：
2011-11-01
影响因子：
3.7
通讯作者：
Chen, Jihua
中科院分区：
文献类型：
--
作者：
Liu, Ruirui;Fang, Ming;Chen, Jihua
将富含原花青素的葡萄籽提取物粉末加入不同浓度的溶剂中，制备了原花青素基预处理剂。将脱矿牙本质标本预处理20、30、60和120 s，然后评价其交联度、力学性能和微观形态。预处理后脱矿牙本质胶原的交联度呈浓度和时间依赖性增加，与预处理剂和溶剂无关。当处理相同的暴露时间，标本后15%的原花青素预处理导致最高的平均极限拉伸强度相比，所有其他组测试。5%戊二醛对照组交联度最高，但拉伸强度低于15%原花青素对照组。场发射扫描电镜证实，脱矿牙本质胶原蛋白在预处理后呈均匀、规则的排列，并保持膨胀，而与表面湿度条件无关。
Proanthocyanidin-based preconditioners were prepared by adding powdered proanthocyanidins-rich grape seed extract to various solvents at different concentrations. Demineralized dentin specimens were preconditioned for 20, 30, 60 or 120 s, followed by the evaluation of their cross-linking degree, mechanical properties and micromorphology. The cross-linking degree of the demineralized dentin collagen exhibited concentration-and time-dependent increase after preconditioning treatment, irrespective of the preconditioner and the solvent. When treated for the same exposure time, specimens after 15% proanthocyanidins preconditioning resulted in the highest mean ultimate tensile strength compared with all the other groups tested. Five percent glutaraldehyde control group produced the highest cross-linking degree, but the ultimate tensile strength was lower than that of 15% proanthocyanidins group. The field emission scanning electron microscopy confirmed that the demineralized dentin collagen was in a homogeneous and regular arrangement after preconditioning and maintained expanding, regardless of the surface moisture conditions.