The β-adrenoceptor agonist clenbuterol is a potent inhibitor of the LPS-induced production of TNF-α and IL-6 in vitro and in vivo
The β-adrenoceptor agonist clenbuterol is a potent inhibitor of the LPS-induced production of TNF-α and IL-6 in vitro and in vivo
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DOI：
10.1007/s000110050493
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发表时间：
1999-09-01
影响因子：
6.7
通讯作者：
Witkamp, RF
中科院分区：
文献类型：
--
作者：
Izeboud, CA;Monshouwer, M;Witkamp, RF
目标与设计：研究β-激动剂克仑特罗在体内外对脂多糖（LPS）炎症模型中TNF-α和IL-6释放的抑制作用。材料和对象：人U-937细胞系（单核细胞衍生的巨噬细胞）和雄性Wistar大鼠处理：将U-937巨噬细胞与1 μ g/ml的LPS一起孵育1 - 24小时，其中含有或不含有1.0mM-0.1nM的测试药物（克伦特罗和其它cAMP升高剂）。大鼠口服1或10 μ g/kg克仑特罗（或生理盐水），1小时前腹腔注射2毫克/公斤LPS.Methods和结果：TNF-α和IL-6的时间-浓度曲线测定在培养基和血浆中，使用ELISA和生物测定。LPS介导的释放两种细胞因子显着抑制由clenbuterol.Conclusions：β-激动剂克伦特罗非常有力地抑制LPS诱导的释放的促炎细胞因子TNF-α和IL-6在体外和体内。
Objective and Design: To investigate the suppressive effects of the beta-agonist clenbuterol on the release of TNF-alpha and IL-6 in a lipopolysaccharide (LPS)-model of inflammation, both in vitro and in vivo.Material and Subjects: Human U-937 cell line (monocyte-derived macrophages), and male Wistar rats (200-250 g).Treatment: U-937 macrophages were incubated with LPS at 1 mu g/ml, with or without 1.0 mM-0.1 nM test drugs (clen-buterol and other cAMP elevating agents) for 1-24 h. Rats were administered either 1 or 10 mu g/kg clenbuterol (or saline) orally, 1 h before intraperitoneal administration of 2 mg/kg LPS.Methods and Results: TNF-alpha and IL-6 time-concentration profiles were determined both in culture media and plasma, using ELISA's and bioassays. LPS-mediated release of both cytokines was significantly suppressed by clenbuterol.Conclusions: The beta-agonist clenbuterol very potently suppresses the LPS-induced release of the pro-inflammatory cytokines TNF-alpha and IL-6 both in vitro and in vivo.