Effect of triterpene acids of Eriobotrya japonica (Thunb.) Lindl. leaf on inflammatory cytokine and mediator induction from alveolar macrophages of chronic bronchitic rats
Effect of triterpene acids of Eriobotrya japonica (Thunb.) Lindl. leaf on inflammatory cytokine and mediator induction from alveolar macrophages of chronic bronchitic rats
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枇杷 (Eriobotrya japonica (Thunb.) Lindl.) 三萜酸的作用。
DOI：
10.1007/s00011-006-5185-0
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发表时间：
2007-02-01
影响因子：
6.7
通讯作者：
Wang, Y. -Y.
中科院分区：
文献类型：
--
作者：
Huang, Y.;Li, J.;Wang, Y. -Y.
目的：研究枇杷叶三萜酸类化合物对枇杷果实的抗衰老作用。Lindl.方法气管内注入脂多糖（LPS）诱导慢性支气管炎（CB）大鼠，1周后尾静脉注射卡介苗（BCG）诱导CB。给药组以不同剂量（50、150或450 mg/kg，每日i. g.），富马酸酮替芬（5 mg/kg，每日i. g.）或地塞米松（1.2mg/kg，每日i. g.）注射LPS后7天，共2周。然后分离AM并孵育24小时。用胸腺细胞共刺激法和放射免疫法测定培养上清中IL-1、TNF-α和PGE 2的含量。免疫细胞化学染色和Western-blot检测NF-κ B p65亚基在细胞内的定位和活化。结果与CB组相比，TAL组AM中TNF-α、IL-1、NF-κ B、PGE 2和LTB 4的表达水平明显降低结论TAL可抑制CB大鼠AM NF-κ B的活化，下调AM中TNF-α、IL-1、PGE 2和LTB 4表达的变化，这可能是其抗炎作用的机制之一。
ObjectiveThe study was to evaluate the effect of triterpene acids ofEriobotrya japonica(thunb.)lindl. leaf (TAL) on inflammatory cytokine and mediator expression in alveolar macrophages (AM) of chronic bronchitic (CB) rats.MethodsCB was induced by endotracheal instillation of lipopolysaccharide (LPS) followed by Bacillus Calmette Guerin (BCG) injection via the caudal vein one week later. Treatment groups received TAL at there different doses (50, 150, or 450 mg/kg daily i. g.), Ketotifen fumarate (5 mg/kg daily i. g.) or dexamethasone (1.2 mg/kg daily i. g.) for two weeks, 7 days after LPS injection. AM were then isolated and incubated for 24 h. IL-1, TNF-α and PGE2 levels in cultured supernatants were measured by thymocyte co-stimulating assay and radioimmunoassay. Immunocytochemistry staining and western-blot were used for intracellular location and activation of p65 subunit of NF-kB. LTB4level was analyzed by reverse-phase high performance liquid chromatography (RP-HPLC).ResultsThe levels of TNF-α, IL-1, NF-kB, PGE2 and LTB4expression in AM of TAL groups were significantly decreased compared to the CB group (P< 0.05 orP< 0.01), in a dose dependent manner.ConclusionTAL inhibited NF-kB activation in AM from CB rats and led to down regulation of TNF-α, IL-1, PGE2and LTB4expression, which might be a mechanism for its anti-inflammatory effects in CB rats.