1,25,26-trihydroxyvitamin D3: isolation, identification, and biological activity.
1,25,26-trihydroxyvitamin D3: isolation, identification, and biological activity.
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1,25,26-三羟基维生素 D3：分离、鉴定和生物活性。
DOI：
10.1016/0003-9861(81)90169-7
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发表时间：
1981
影响因子：
3.9
通讯作者：
DeLuca,HF
中科院分区：
文献类型：
--
作者：
Tanaka,Y;Schnoes,HK;Smith,CM;DeLuca,HF
从补充维生素d的鸡的肾脏匀浆中培养1,25-二羟基维生素d3，或从缺乏维生素d的鸡的肾脏匀浆中培养2526 -二羟基维生素d3，在体外产生了维生素d3的极性代谢物。该化合物经紫外吸收分光光度法和质谱法分离纯化，鉴定为1,25,26-三羟基维生素d3。在高效液相色谱上，其高碘酸盐裂解产物与合成的1α-羟基-25-酮-27-去甲维生素d3相匹配。1,2526 -三羟基维生素d3在刺激肠钙运输和骨钙动员方面的活性是1,25-二羟基维生素d3的0.1-0.01。
A polar metabolite of vitamin D3has been producedin vitrofrom either 1,25-dihydroxyvitamin D3incubated with kidney homogenate from vitamin D-supplemented chickens or from 25,26-dihydroxyvitamin D3incubated with vitamin D-deficient chicken kidney homogenate. This compound was isolated in pure form and identified as 1,25,26-trihydroxyvitamin D3by ultraviolet absorption spectrophotometry and mass spectrometry. Furthermore, its periodate cleavage product comigrates with synthetic 1α-hydroxy-25-keto-27-norvitamin D3on high-performance liquid chromatography. The 1,25,26-trihydroxyvitamin D3is 0.1-0.01 as active as 1,25-dihydroxyvitamin D3in the stimulation of intestinal calcium transport and bone calcium mobilization.