Post-traumatic survival and recovery of the auditory sensory cells in culture
Post-traumatic survival and recovery of the auditory sensory cells in culture
复制标题
DOI：
10.3109/00016489609137836
复制
发表时间：
1996-03-01
影响因子：
1.4
通讯作者：
Slapnick, SM
中科院分区：
文献类型：
--
作者：
Sobkowicz, HM;August, BK;Slapnick, SM
在器官型培养物中机械损伤后，听毛细胞显示出存活和最初改革其顶端特化、表皮板和静纤毛的能力，但没有显示出掺入氚化胸苷（有丝分裂标记物）的能力。网状层的破坏和毛细胞表皮板的局部损伤诱导支持细胞的增殖。内毛细胞的再生尖被内螺旋沟细胞和内指细胞包裹，外毛细胞的再生尖被Deiters细胞和外螺旋沟细胞的指突包裹。这些毛细胞中的一些随后重新出现新形成的顶部。失去与器官表面接触的毛细胞仍然深埋在上皮细胞中，但仍然活着。我们的研究提供的证据表明，哺乳动物的Corti器官对损伤的反应不是通过形成新的感觉细胞，而是通过恢复先前存在的有丝分裂后的毛细胞。
Following mechanical injury in organotypic cultures, auditory hair cells show the ability to survive and to initially reform their apical specializations, cuticular plates and stereocilia, but none show incorporation of tritiated thymidine, the mitotic marker. Disruption of the reticular lamina and local injury to hair cell cuticular plates induces proliferation of supporting cells. The regenerating apices of inner hair cells are wrapped by the cells of the inner spiral sulcus and the inner phalangeal cells, while those of outer hair cells are wrapped by the phalangeal processes of Deiters' cells and outer spiral sulcus cells. Some of these hair cells subsequently resurface with newly formed tops. Hair cells that lose contact with the surface of the organ remain buried-but alive-deep within the epithelium. Our study provides evidence that the mammalian organ of Corti responds to injury not by the formation of new sensory cells but by the recovery of the pre-existing postmitotic hair cells.