Multiple components of endo-polyguluronide lyase of Pseudomonas sp.
Multiple components of endo-polyguluronide lyase of Pseudomonas sp.
复制标题
假单胞菌内聚古洛糖酸裂解酶的多种成分。
DOI：
10.1093/oxfordjournals.jbchem.a131488
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发表时间：
1977
影响因子：
2.7
通讯作者：
K. Nisizawa
中科院分区：
文献类型：
--
作者：
K. Min;S. Sasaki;Y. Kashiwabara;H. Suzuki;K. Nisizawa
从超声处理的假单胞菌属（Pseudomonassp.）细胞的透析提取物获得的冻干藻酸盐裂解酶制剂通过凝胶过滤在Sephadex G-150柱上分级分离，并且获得三个藻酸盐裂解酶[EC 4.2.2.3]级分，峰I、II和III。它们非常不耐热。裂解酶组分降解两种藻酸盐片段，聚葡萄糖醛酸苷（SG）和由甘露糖醛酸和古洛糖醛酸残基（SMG）组成的聚葡萄糖醛酸苷，以及藻酸盐，但对聚甘露糖醛酸苷片段（SM）几乎没有活性。裂解酶组分降解这些底物的模式具有不同程度的随机性。峰I馏分的攻击比峰II和峰III的攻击更随机。SG和SMG通过这些酶形成的主要裂解产物被鉴定为不饱和三脲和单脲的混合物。来自SG的不饱和三糖醛酸苷是deltaUGG，SMG产生deltaUGG和表征不佳的不饱和三聚体（可能是deltaUMG）的混合物。然而，在孵育过程中，这些酶组分产生单体和三聚体的模式以不同的方式发生变化。
A lyophilized alginate lyase preparation obtained from dialyzed extract of sonicated Pseudomonas sp. cells was fractionated by gell filtration on a Sephadex G-150 column, and three alginate lyase [EC4.2.2.3] fractions, peaks I, II, and III, were obtained. They were remarkably thermolabile. The lyase fractions degraded two kinds of alginate fragments, a polygluuronide (SG), and a polyuronide consisting of both mannuronic and guluronic acid residues (SMG), as well as commerical alginate, but were virtually inactive toward polymannuronide fragment (SM). The modes of degradation of these substrates by the lyase fractions were endowise with different degrees of randomness. Attack by the peak I fraction was more random than those by peaks II and III. The main lysis products formed from SG and SMG by these layses were identified as mixtures of unsaturated tri- and monouronides. The unsaturated triuronide from SG was deltatugg and SMG yielded a mixture of deltaUGG and a poorly characterized unsaturated trimer, possibly deltaUMG. However, the patterns of monomer and trimer production by theselyase fractions changed in different ways during incubation.