1H-NMR studies at 500 MHz of a neutral disaccharide and sulphated di-, tetra-, hexa- and larger oligosaccharides obtained by endo-beta-galactosidase treatment of keratan sulphate.
1H-NMR studies at 500 MHz of a neutral disaccharide and sulphated di-, tetra-, hexa- and larger oligosaccharides obtained by endo-beta-galactosidase treatment of keratan sulphate.
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在 500 MHz 下对中性二糖和硫酸化二糖、四糖、六糖和更大的寡糖进行 1H-NMR 研究，这些寡糖是通过内切 β-半乳糖苷酶处理硫酸角质素获得的。
DOI：
10.1111/j.1432-1033.1986.tb09679.x
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
T. Feizi
中科院分区：
文献类型：
--
作者：
E. Hounsell;J. Feeney;P. Scudder;P. Tang;T. Feizi
在该杂志的前一篇论文中，通过内切β-半乳糖苷酶消化牛角膜硫酸角质素获得的主要寡糖被鉴定为中性二糖GlcNAc β 1-3Gal，以及基于序列（-3/4GlcNAc β 1-3Gal β 1-）n分别具有1、3、5、7和9个硫酸基团的硫酸化二糖、四糖、六糖、八糖和十糖。在本研究中，这些寡糖已被分析500 MHz的1H-NMR光谱使用自旋去耦和二维相关光谱实验。NMR数据证实了所有糖苷间键的β-构型，并且与-4GlcNAc和-3Gal、非还原端N-乙酰葡糖胺残基和还原端半乳糖残基的交替序列一致。NMR数据还证实，硫酸根基团与除还原端半乳糖外的所有糖残基的C6位置连接，如下所示：（分子式：见正文）。与硫酸化碳原子连接的质子的信号显示出明显的低场位移（相对于非硫酸化碳原子的当量质子约0.4ppm），而与硫酸化原子相邻的C5处的质子显示出0.1- 0.2ppm的变化，硫酸化单糖的其他质子显示出较小的化学位移变化（0.01- 0.1ppm）。在C3处连接的非硫酸化还原末端半乳糖的C4处的质子也显示出化学位移的显著变化（0.03ppm）。
In the preceding paper in this journal, the major oligosaccharides obtained by endo-beta-galactosidase digestion of bovine corneal keratan sulphate were identified as a neutral disaccharide, GlcNAc beta 1-3Gal, and sulphated di-, tetra-, hexa-, octa- and decasaccharides based on the sequence (-3/4GlcNAc beta 1-3Gal beta 1-)n having 1, 3, 5, 7 and 9 sulphate groups, respectively. In the present study, these oligosaccharides have been analysed by 500-MHz 1H-NMR spectroscopy using spin-decoupling and two-dimensional correlated spectroscopy experiments. The NMR data confirm the beta-configuration of all the interglycosidic linkages and are consistent with an alternating sequence of----4GlcNAc and----3Gal, a non-reducing-end N-acetylglucosamine residue and a reducing-end galactose residue. The NMR data have also established that a sulphate group is linked to the C6 position of all sugar residues except the reducing-end galactose as follows: (Formula: see text). The signals of the protons attached to the sulphated carbon atoms show marked downfield shifts (approximately 0.4 ppm from equivalent protons of non-sulphated carbon atoms), while the protons at C5 vicinal to sulphated atoms show a change of 0.1-0.2 ppm and other protons of the sulphated monosaccharides show smaller changes in chemical shift (0.01-0.1 ppm). The proton at C4 of the non-sulphated reducing-end galactose linked at C3 also shows a significant change in chemical shift (0.03 ppm).
兔角膜基质硫酸蛋白角质素的单克隆抗体。
影响因子：
2
作者：
SundarRaj,N;Willson,J;Gregory,JD;Damle,SP
通讯作者：
Damle,SP
鉴定特异性识别角膜和骨骼硫酸角质素的单克隆抗体。
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Caterson,B;Christner,JE;Baker,JR
通讯作者：
Baker,JR