Effect of norcantharidin on proliferation and invasion of human gallbladder carcinoma GBC-SD cells
Effect of norcantharidin on proliferation and invasion of human gallbladder carcinoma GBC-SD cells
复制标题
DOI：
10.3748/wjg.v11.i16.2431
复制
发表时间：
2005-04-28
影响因子：
4.3
通讯作者：
Chen, Cun-Qiu
中科院分区：
文献类型：
--
作者：
Fan, Yue-Zu;Fu, Jin-Ye;Chen, Cun-Qiu
目的：探讨去甲斑蝥素对人胆囊癌GBC-SD细胞体外增殖、侵袭的影响及其抗癌机制。方法：采用细胞培养技术培养人胆囊癌GBC-SD细胞。采用四唑比色法、基质胶实验和过河实验评价GBC-SD细胞的体外生长和侵袭能力。采用链霉亲和素-生物素复合物法测定GBC-SD细胞PCNA、Ki-67、MMP2和TIMP2蛋白的表达。结果：体外去甲斑蝥素对GBC-SD细胞的生长和增殖具有剂量和时间依赖性的抑制作用，48 h时的IC50值为56.18 μg/mL。去甲斑蝥素在5μg/mL浓度时开始抑制GBC-SD细胞的侵袭，40μg/mL浓度时GBC-SD细胞的侵袭作用被完全抑制，过河时间显着延长。去甲斑蝥素处理后，PCNA、Ki-67和MMP2的表达显着降低。随着TIMP2表达的增加，MMP2与TIMP2的比率显着降低（P
AIM: To investigate the effect of norcantharidin on proliferation and invasion of human gallbladder carcinoma GBC-SD cells in vitro and its anticancer mechanism.METHODS: Human gallbladder carcinoma GBC-SD cells were cultured by cell culture technique. The growth and the invasiveness of GBC-SD cells in vitro were evaluated by the tetrazolium-based colorimetric assay and by the Matrigel experiment and the crossing-river test. Expression of PCNA, Ki-67, MMP2 and TIMP2 proteins of GBC-SD cells was determined by streptavidin-biotin complex method.RESULTS: In vitro norcantharidin inhibited the growth and proliferation of GBC-SD cells in a dose-and time-dependent manner, with the IC50 value of 56.18 mu g/mL at 48 h. Norcantharidin began to inhibit the invasion of GBC-SD cells at the concentration of 5 mu g/mL, and the invasive action of GBC-SD cells was inhibited completely and their crossing-river time was prolonged significantly at 40 mu g/mL. After treatment with norcantharidin, the expression of PCNA, Ki-67, and MMP2 was significantly decreased. With the increase in TIMP2 expression, the MMP2 to TIMP2 ratio was decreased significantly (P