Inheritance of AFLP markers and their use for genetic diversity analysis in wild and farmed scallop (Chlamys farreri)
Inheritance of AFLP markers and their use for genetic diversity analysis in wild and farmed scallop (Chlamys farreri)
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DOI：
10.1016/j.aquaculture.2008.10.033
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发表时间：
2009-02
期刊：
影响因子：
4.5
通讯作者：
Cui Zhao;Qi Li;L. Kong
中科院分区：
文献类型：
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作者：
Cui Zhao;Qi Li;L. Kong
对栉孔扇贝3个全同胞家系的AFLP表型遗传模式进行了研究。总体而言，在Bonferroni校正之前，9.3%的分析显示出非孟德尔分离比，校正后有0.8%显着，这表明AFLP标记可以被认为是孟德尔性状。利用AFLP分子标记技术研究了中国对虾养殖群体的遗传多样性水平。并将其与野生种群进行比较。6对引物在4个养殖群体和2个野生群体的139个个体中共扩增出293个位点。在AFLP标记上，养殖和野生红腹锦鸡儿群体均表现出较高的多态性。farreri populations.期望杂合度范围为0.291（孵化）和0.295（野生），而多态位点的百分比范围为92.1%（孵化）和93.9%（野生），分别。这表明，30多年的人工养殖并没有实质上降低养殖种群的遗传变异性。法勒里养殖种群之间以及养殖种群与野生种群之间存在显著的遗传分化。从这项研究中获得的信息是有用的，为未来的遗传改良选择育种，并设计合适的管理准则，这些遗传材料。
Inheritance patterns of amplified fragment length polymorphism (AFLP) phenotypes were tested in three full-sib families of Zhikong scallop Chlamys farreri. Overall, 9.3% of analyses exhibited non-Mendelian segregation ratios before Bonferroni correction, with 0.8% significant after correction, demonstrating that AFLP markers can be considered Mendelian traits. AFLP markers were used to investigate levels of genetic diversity within cultured stocks of C. farreri and to compare them with the wild populations. Six pairs of primers generated 293 loci among 139 individuals in four cultured and two wild populations. High polymorphism at the AFLP markers was found within both cultured and wild C. farreri populations. The expected heterozygosity ranged from 0.291 (hatcheries) to 0.295 (wild), while the percentage of polymorphic loci ranged from 92.1% (hatcheries) to 93.9% (wild), respectively. This suggests that more than 30 years of artificial cultivation have not substantially reduced the genetic variability of the cultured stocks of C. farreri. Significant genetic differentiation was observed between the cultured stocks, and between the cultured and wild populations. The information derived from this study is useful for future genetic improvement by selective breeding, and for designing suitable management guidelines for these genetic materials.