FORMATION OF PROTEINASE INHIBITORS IN DEVELOPING BARLEY GRAIN
FORMATION OF PROTEINASE INHIBITORS IN DEVELOPING BARLEY GRAIN
复制标题
DOI：
10.1111/j.1399-3054.1973.tb03081.x
复制
发表时间：
1973-01-01
影响因子：
6.4
通讯作者：
KIRSI, M
中科院分区：
文献类型：
--
作者：
KIRSI, M
对大麦籽粒发育过程中胰酶抑制剂和曲霉-蛋白酶抑制剂的活性进行了测定。在可控制的条件下生长的。胰酶抑制剂活性在发育早期出现，在开花后24天达到接近最大值，但曲霉-蛋白水解酶抑制剂的合成在发育相当晚。开花后25d左右达到半最大值。所有蛋白质合成的半峰时间约为16d。发育中的籽粒中存在的胰酶抑制剂与成熟籽粒中的胚乳抑制剂在免疫学上是一致的。不同品种大麦的曲霉-蛋白水解酶抑制剂的浓度存在差异。此外，每个品种的抑制活性与总蛋白含量的增加几乎呈线性关系。不同品种之间的胰酶抑制活性也存在较大差异。然而，每个品种的活性是恒定的，与总蛋白质含量无关。作者非常感谢Juhani Mikola博士在工作过程中进行了许多有益的讨论，并就手稿提出了几个有价值的建议。我还要感谢Marja-Leena Niku-Paavola夫人，Lic。Phil.感谢在免疫扩散实验中的宝贵帮助，Outi Karkkainen小姐提供熟练的技术帮助。这项工作得到了埃米尔·阿尔托宁基金会和E·J·萨里奥拉基金会的赠款支持。
Assays have been made of the activities of trypsin inhibitors and Aspergillus‐proteinase inhibitors in developing grains of barley (Hordeum vulgare L.) grown under controlled conditions. Trypsin inhibitor activity appeared early in the development, reaching a near‐maximal value 24 days after anthesis, but Aspergillus‐proteinase inhibitors were synthesized quite late in the development. A half‐maximal value was attained about 25 days subsequent to anthesis. The corresponding half‐maximal value for all the proteins synthesized was about 16 days.The trypsin inhibitor present in developing grains appeared to be immunologically identical with the endospermal inhibitor in mature grains.The concentrations of Aspergillus‐proteinase inhibitors varied between different cultivars of barley. Moreover, each cultivar displayed an almost linear relationship between inhibitor activity and the increasing content of total protein. Great differences between different cultivars were also observable with regard to trypsin inhibitor activities. However, the activities in each cultivar were constant, regardless of the total protein content.The author is greatly indebted to Dr. Juhani Mikola for many helpful discussions during the course of the work, and for several valuable suggestions in regard to the manuscript. I also wish to thank Mrs. Marja‐Leena Niku‐Paavola, Lic. Phil., for valuable help in the immunodiffusion experiments, and Miss Outi Karkkainen for skilful technical assistance. This work was supported by grants from the Emil Aaltonen Foundation and from the E. J. Sariola Foundation.