Nucleic Acid Templated Reactions for Chemical Biology.
Nucleic Acid Templated Reactions for Chemical Biology.
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DOI：
10.1002/cmdc.201700266
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发表时间：
2017-06-21
期刊：
影响因子：
3.4
通讯作者：
Seitz O
中科院分区：
文献类型：
--
作者：
Di Pisa M;Seitz O
核酸导向的生物正交反应提供了一个迷人的机会，揭示和重定向过多的细胞内机制。纳米至皮摩尔量的特定RNA分子作为模板，催化分子的选择性形成，1）发挥生物学效应，或2）为RNA检测提供可测量的信号。 当RNA模板的浓度低时，模板上的反应物的周转是有价值的资产。其想法是使用RNA模板反应来完全控制药物的生物分布，并将DNA或RNA分析物的检测限提高到非常高的灵敏度。在这里，我们回顾了最近的和有指导意义的例子，有条件的合成或释放的化合物，在cellulo蛋白质干扰和细胞内核酸成像。
Nucleic acid directed bioorthogonal reactions offer the fascinating opportunity to unveil and redirect a plethora of intracellular mechanisms. Nano‐ to picomolar amounts of specific RNA molecules serve as templates and catalyze the selective formation of molecules that 1) exert biological effects, or 2) provide measurable signals for RNA detection. Turnover of reactants on the template is a valuable asset when concentrations of RNA templates are low. The idea is to use RNA‐templated reactions to fully control the biodistribution of drugs and to push the detection limits of DNA or RNA analytes to extraordinary sensitivities. Herein we review recent and instructive examples of conditional synthesis or release of compounds for in cellulo protein interference and intracellular nucleic acid imaging.