Helicobacter pylori infection aggravates diet-induced nonalcoholic fatty liver in mice
Helicobacter pylori infection aggravates diet-induced nonalcoholic fatty liver in mice
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幽门螺杆菌感染会加重小鼠饮食诱发的非酒精性脂肪肝。
DOI：
10.1016/j.clinre.2017.12.008
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发表时间：
2018-09-01
影响因子：
2.7
通讯作者：
Lu, Nonghua
中科院分区：
文献类型：
--
作者：
He, Cong;Chen, Dandan;Lu, Nonghua
背景：之前的流行病学研究表明幽门螺杆菌（H. pylori）感染与非酒精性脂肪肝（NAFLD）之间存在联系，但动物研究正在努力阐明这种联系。在本研究中，我们评估了幽门螺杆菌感染对小鼠NAFLD的潜在影响。方法：我们首先建立了两种幽门螺杆菌感染小鼠模型，分别采用普通饮食和高脂饮食（HFD）。测量身体和肝脏重量、血糖、血清转氨酶和脂质水平以及肝脏炎症标志物。还对肝组织进行了组织学分析。还测定了脂肪合成基因以及胰岛素信号蛋白的表达。结果：治疗24周后，与HFD对照组相比，感染幽门螺杆菌SS1的HFD喂养小鼠的腹围、空腹血糖、低密度胆固醇和丙氨酸转氨酶显着增加。此外，用 HFD 喂养的感染幽门螺杆菌 SS1 的小鼠表现出明显更多的肝脏脂肪变性。 H. pylori SS1感染抑制了IRS1和Akt的磷酸化，并有增加肝脏中IL-1β和TNF-α表达的趋势。结论：H. pylori感染与C57BL/6小鼠的NAFLD相关，具体取决于菌株和饮食结构。幽门螺杆菌SS1而非NCTC11637的感染与HFD联合诱导了更严重的肝脏脂肪变性。幽门螺杆菌感染可能在 NAFLD 的发展中发挥作用，需要进一步研究以确定根除幽门螺杆菌是否可以提高 NAFLD 风险。 (C) 2018 年由 Elsevier Masson SAS 出版。
Background: Previous epidemiological studies have suggested a link between Helicobacter pylori (H. pylori) infection and nonalcoholic fatty liver disease (NAFLD), yet animal studies are tacking to elucidate this association. In this study, we evaluated the potential effects of H. pylori infection on NAFLD in mice.Methods: We first established two strains of H. pylori infected mice model with either chow diet or high fat diet (HFD). The body and liver weight, blood glucose, serum transaminases and lipid levels and markers of hepatic inflammation were measured. Histological analyses were also performed on liver tissue. Expressions of fat synthesis genes as well as insulin signaling proteins were also determined.Results: After 24 weeks of treatment, the abdominal circumference, fasting blood glucose, low-density cholesterol and alanine transaminase were significantly increased in HFD feeding mice infected with H. pylori SS1 compared to HFD controls. Moreover, HFD fed mice infected with H. pylori SS1 showed significantly more liver steatosis. H. pylori SS1 infection inhibited phosphorylation of IRS1 and Akt and trended to increase the expression of IL-1 beta and TNF-alpha in the liver.Conclusion: H. pylori infection is associated with NAFLD in C57BL/6 mice which depends on the bacterial strain and diet structure. The infection of H. pylori SS1 instead of NCTC11637 in combination with HFD induced more severe liver steatosis. H. pylori infection may play a role in NAFLD development and further studies are needed to determine whether H. pylori eradication can improve NAFLD risk. (C) 2018 Published by Elsevier Masson SAS.