Multispectral imaging in the extended near-infrared window based on endogenous chromophores
Multispectral imaging in the extended near-infrared window based on endogenous chromophores
复制标题
DOI：
10.1117/1.jbo.18.10.101318
复制
发表时间：
2013-10-01
影响因子：
3.5
通讯作者：
Berezin, Mikhail Y.
中科院分区：
文献类型：
--
作者：
Cao, Qian;Zhegalova, Natalia G.;Berezin, Mikhail Y.
为了在增加穿透深度的同时最大限度地减少深层组织中的散射问题，我们探索了在相对未被探索的扩展近红外(ExNIR)光谱区域使用内源生色团在900至1400 nm处成像的可行性。这一区域，也被称为第二近红外窗口，由来自水和脂类的吸收弱控制，不存在其他内源发色团，几乎没有自发荧光。为了证明exNIR在生物成像中的适用性，我们使用InGaAs分光光度计和具有透射式几何结构的多光谱InGaAs扫描成像器分析了单个组件和生物组织的光学性质。基于组织光谱特性的差异，我们利用比率计量法从获取的三维数据立方体中提取光谱特征。获得的通过小鼠头的exNIR传输的图像显示了与解剖结构一致的足够细节。(C)2013年光学仪器工程师学会(SPIE)
To minimize the problem with scattering in deep tissues while increasing the penetration depth, we explored the feasibility of imaging in the relatively unexplored extended near infrared (exNIR) spectral region at 900 to 1400 nm with endogenous chromophores. This region, also known as the second NIR window, is weakly dominated by absorption from water and lipids and is free from other endogenous chromophores with virtually no autofluorescence. To demonstrate the applicability of the exNIR for bioimaging, we analyzed the optical properties of individual components and biological tissues using an InGaAs spectrophotometer and a multispectral InGaAs scanning imager featuring transmission geometry. Based on the differences in spectral properties of tissues, we utilized ratiometric approaches to extract spectral characteristics from the acquired three-dimensional "datacube". The obtained images of an exNIR transmission through a mouse head revealed sufficient details consistent with anatomical structures. (C) 2013 Society of Photo-Optical Instrumentation Engineers (SPIE)