Related 5S RNA transcription factors in Xenopus oocytes and somatic cells.
Related 5S RNA transcription factors in Xenopus oocytes and somatic cells.
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非洲爪蟾卵母细胞和体细胞中相关的 5S RNA 转录因子。
DOI：
10.1073/pnas.78.3.1760
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发表时间：
1981
影响因子：
11.1
通讯作者：
Brown,DD
中科院分区：
文献类型：
--
作者：
Pelham,HR;Wormington,WM;Brown,DD
非洲爪蟾卵母细胞含有丰富的蛋白质，该蛋白质特异性地结合到5S RNA基因的中心，并通过RNA聚合酶III指导其转录。该蛋白质还与5S RNA结合。我们在这里表明，克隆的5S RNA基因的转录在提取物中来自非洲爪蟾组织培养细胞是依赖于基因内控制区，并抑制5S RNA和抗体提出对以前的特点卵母细胞转录因子。体细胞含有一种蛋白质，其与卵母细胞因子的作用、对肝素-琼脂糖的亲和力和抗原性相似，但其表观分子量比卵母细胞蛋白质大约2000道尔顿。我们的实验强烈表明，这种较大的蛋白质是体细胞中5S RNA基因的转录因子。5S RNA可以通过与这种蛋白质结合来调节其自身在体细胞中的合成，这种蛋白质以低浓度存在。在卵母细胞和体细胞中负责5S RNA合成的两种不同蛋白的存在本身不能解释卵母细胞和体细胞5S RNA基因的发育控制，因为体细胞提取物转录两种类型的基因。
Xenopus oocytes contain an abundant protein that binds specifically to the center of 5S RNA genes and directs their transcription by RNA polymerase III. This protein also binds to 5S RNA. We show here that transcription of cloned 5S RNA genes in extracts derived from Xenopus tissue culture cells is dependent on the intragenic control region and is inhibited by 5S RNA and by antibodies raised against the previously characterized oocyte transcription factor. Somatic cells contain a protein that is similar to the oocyte factor in charge, affinity for heparin-agarose, and antigenicity but has an apparent molecular mass about 2000 daltons greater than that of the oocyte protein. Our experiments strongly suggest that this larger protein is the transcription factor for 5S RNA genes in somatic cells. The 5S RNA may regulate its own synthesis in somatic cells by binding to this protein, which is present at a low concentration. The presence of two different proteins responsible for 5S RNA synthesis in oocytes and in somatic cells cannot by itself explain the developmental control of oocyte and somatic 5S RNA genes, because somatic cell extracts transcribe both types of gene.