Molecular analysis of the fur (ferric uptake regulator) gene of a pathogenic Edwardsiella tarda strain
Molecular analysis of the fur (ferric uptake regulator) gene of a pathogenic Edwardsiella tarda strain
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DOI：
10.1007/s12275-008-0038-x
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发表时间：
2008-07
期刊：
影响因子：
--
通讯作者：
F. Wang;Shuang Cheng;Kun Sun;Li Sun
中科院分区：
文献类型：
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作者：
F. Wang;Shuang Cheng;Kun Sun;Li Sun
从一种病原体中克隆出了爱德华氏菌（edwardsiella tardaferric uptake regulator, FurEt）的编码基因。从病鱼中分离的缓步虫。furett与大肠杆菌（FurEc）有90%的序列一致性，并且能够补充afurec -缺陷vee的突变表型。colistrain。突变分析表明，C92S和C95S突变使furet变异体失活，而E112K突变导致furet变异体超活性。furett负向调控自身表达；这种调节的中断会损害细菌的生长，改变某些外膜蛋白的产生，并减弱细菌的毒力。
The gene encoding theEdwardsiella tardaferric uptake regulator (FurEt) was cloned from a pathogenicE. tardastrain isolated from diseased fish. FurEtshares 90% overall sequence identity with theEscherichia coliFur (FurEc) and was able to complement the mutant phenotype of afurEc-defectiveE. colistrain. Mutational analysis indicated that C92S and C95S mutations inactivated FurEtwhereas E112K mutation resulted in a superactive FurEtvariant. FurEtnegatively regulated its own expression; interruption of this regulation impaired bacterial growth, altered the production of certain outer membrane proteins, and attenuated bacterial virulence.