Construction and characterization of a poliovirus/rhinovirus antigenic hybrid.
Construction and characterization of a poliovirus/rhinovirus antigenic hybrid.
复制标题
脊髓灰质炎病毒/鼻病毒抗原杂交体的构建和表征。
DOI：
10.1016/0042-6822(91)90433-c
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发表时间：
1991
期刊：
影响因子：
3.7
通讯作者：
Wimmer,E
中科院分区：
文献类型：
--
作者：
Altmeyer,R;Murdin,AD;Harber,JJ;Wimmer,E
为了研究表达在脊髓灰质炎病毒上的外源抗原位点的性质，构建了以人鼻病毒14株(HRV14)的中和免疫位点IA(NImIA)取代脊髓灰质炎病毒1型毒株的中和抗原位点IA[PV1(M)]的杂交体。由此产生的杂交是可行的，但与PV1(M)相比，生长受到了损害。该杂交种同时表达PV1(M)和HRV14抗原决定簇。当接种到兔体内时，可诱导出针对PV1(M)和HRV14的中和抗体。此外，针对PV1(M)或HRV14的多克隆抗血清以及针对NImIA的5个单抗中的3个都能有效地中和杂交种。单抗还阻止了杂交体与脊髓灰质炎病毒的细胞受体的结合。其中一种被认为可以以这种方式中和鼻病毒，而且似乎NImIA在杂交体上的表达环境足够有利，相同的机制才能有效。这可以被解释为亲本病毒和它们各自的细胞受体之间的相互作用非常相似。
In order to study the properties of foreign antigenic sites expressed on poliovirus a hybrid was constructed in which neutralization antigenic site IA of poliovirus type 1 strain Mahoney [PV1(M)] was replaced by neutralization immunogenic site IA (NImIA) of human rhinovirus 14 (HRV14). The resulting hybrid was viable, but growth was impaired in comparison to PV1(M). The hybrid expressed both PV1(M) and HRV14 antigenic determinants. When inoculated into rabbits it elicited neutralizing antibodies against both PV1(M) and HRV14. Furthermore, the hybrid was efficiently neutralized by polyclonal antisera specific for either PV1(M) or HRV14 and by three out of five monoclonal antibodies directed to NImIA. The monoclonal antibodies also blocked binding of the hybrid to the cellular receptor for poliovirus. One of them is thought to neutralize rhinovirus in this manner, and it appears that NImIA is expressed in a sufficiently favorable context on the hybrid for the same mechanism to be effective. This can be interpreted to mean that the interactions between the parental viruses and their respective cellular receptors are very similar.