Construction of a series of several self‐cleaving RNA duplexes using synthetic 21‐mers
Construction of a series of several self‐cleaving RNA duplexes using synthetic 21‐mers
复制标题
使用合成 21 聚体构建一系列几种自切割 RNA 双链体
DOI：
10.1016/0014-5793(88)80004-8
复制
发表时间：
1988
期刊：
影响因子：
3.5
通讯作者：
E. Ohtsuka
中科院分区：
文献类型：
--
作者：
M. Koizumi;S. Iwai;E. Ohtsuka
Two fragments (21-mers) containing consensus sequences for the self-cleavage domain in transcripts of satellite DNA of the newt were chemically synthesized and found to be cleaved in the presence of Mg2+. The cleaved product contained the 3′-terminal 2′,3′-cyclic phosphate. Twenty-five combinations of partially double-stranded 21-mer RNA which contained different bases within the consensus sequences and at the cleavage sites were tested for self-cleavage. It seemed that guanosine 3′-phosphate was not susceptible to transesterification at the cleavage site.
