Immunohistochemical Localization of Cystic Fibrosis Transmembrane Conductance Regulator in Human Fetal Airway and Digestive Mucosa
Immunohistochemical Localization of Cystic Fibrosis Transmembrane Conductance Regulator in Human Fetal Airway and Digestive Mucosa
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人胎儿气道和消化粘膜囊性纤维化跨膜电导调节器的免疫组织化学定位
DOI：
10.1203/00006450-199408000-00002
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发表时间：
1994
影响因子：
3.6
通讯作者：
É. Puchelle
中科院分区：
文献类型：
--
作者：
D. Gaillard;S. Ruocco;A. Lallemand;W. Dalemans;J. Hinnrasky;É. Puchelle
摘要：用免疫组织化学方法研究了囊性纤维化跨膜传导调节因子（CFTR）在人胎儿消化道呼吸道粘膜的细胞分布。链霉亲和素-生物素免疫过氧化物酶法应用于石蜡包埋标本收集的正常胎儿从7至39周的妊娠。到第7周，CFTR蛋白在卵黄囊中被强烈地检测到，而在肠和气道的未分化上皮中染色较弱。在23周，肠表现出强烈的和弥漫性染色的肠上皮细胞和基础的细胞质反应的第一分泌细胞。在发育过程中，只有轻微的变化，可以检测到CFTR的消化上皮分布。在气道中，CFTR分布遵循头尾成熟。在气管纤毛细胞中，CFTR蛋白在细胞质中弥漫检测早在7周。24-25周后，CFTR也存在于集合管和气道腺体中，主要在腺泡周围。我们的数据表明，CFTR早在器官发生期间7周就存在，并且可能在胎儿生命中发挥重要作用。在气道发育过程中CFTR的分布有一个演变，而在肠道中，CFTR早在22周龄就通过上皮高度表达，并保持相同的分布，直到出生。
ABSTRACT: The cellular distribution of the cystic fibrosis transmembrance conductance regulator (CFTR) in human fetal digestive respiratory mucosa has been studied by immunohistochemistry. The streptavidin-biotin immunoperoxidase method was applied to paraffin-embedded specimens collected from normal fetuses ranging from 7 to 39 wk of gestation. By the 7th wk, CFTR protein was strongly detected in the yolk sack; in contast, the staining was weak in the undifferentiated epithelium of the intestine and the airways. At 23 wk, the intestine showed strongly and diffusely stained enterocytes and a basal cytoplasmic reactivity in the first secretory cells. During development, only slight changes could be detected in the digestive epithelial distribution of CFTR. In the airways, the CFTR distribution followed the cephalocaudal maturation. In the tracheal ciliated cells, the CFTR protein was diffusely detected in the cytoplasm as early as 7 wk. After 24–25 wk, CFTR was also present in the collecting ducts and in the glands of the airways, predominantly in the periphery of the acini. Our data suggest that the CFTR is present as early as 7 wk during organogenesis and probably plays an important role during fetal life. There is an evolution in the CFTR distrubution during airway development whereas in the intestine, CFTR is highly expressed through the epithelium as early as 22 wk and keeps the same distribution until birth.
人胎肺中 CFTR 的表达和 cAMP 介导的液体分泌的存在。
DOI：
10.1152/ajplung.1992.262.4.l472
发表时间：
1992
期刊：
The American journal of physiology
影响因子：
--
作者：
McCrayJr,PB;Reenstra,WW;Louie,E;Johnson,J;Bettencourt,JD;Bastacky,J
通讯作者：
Bastacky,J