Identification of melanoma with a gas sensor array
Identification of melanoma with a gas sensor array
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DOI：
10.1111/j.1600-0846.2007.00284.x
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发表时间：
2008-05-01
影响因子：
2.2
通讯作者：
Di Natale, C.
中科院分区：
文献类型：
--
作者：
D'Amico, A.;Bono, R.;Di Natale, C.
背景/目的：在许多不同的病理学中，特别是在各种癌症中，都发现了疾病与人体散发的空气中化学物质变化之间的关系。癌细胞的代谢在其一生中发生了很大的变化;然后，化学物质的修饰应该在癌组织周围很大。作为一个例子，在这个方向上提供了积极的提示，对肺癌受影响的对象的呼吸成分的研究。除了传统的分析方法，近年来传感器阵列也被应用于这些研究，考虑到化学成分的变化，因为这些变化发生在其他应用中，例如，那些处理食品质量测量。本文首次将传感器阵列应用于研究黑色素瘤和痣之间的区分，即分别对黑色素细胞的恶性和良性影响，提出并讨论了。皮肤表面上病变的定位使得利用差分测量成为可能，差分测量旨在捕获两个相邻皮肤区域之间的差异。这种方法强烈地降低了由于特殊的主观气味背景和皮肤气味可变性而引起的皮肤顶部空间可变性的影响。测量活动涉及40例;其中10例被诊断为黑色素瘤，需手术干预。这些诊断中有九个通过切除组织的组织学检查进一步证实，一个是假阳性。结果：顶空化学成分的差异通过气相色谱调查进行了验证，电子鼻数据的分类提供了与目前使用的诊断仪器相同数量级的估计交叉验证准确度。电子鼻传感器已经显示出对由皮肤损伤释放的挥发性有机化合物具有良好的灵敏度，并且该方法似乎对于恶性病变识别是有效的。本文提出的结果鼓励第二次实验活动，有更多的参与者和系统使用气相色谱质谱技术，以确定一些可能的黑色素瘤生物标志物。
Background/purpose: The relationship between diseases and alterations of the airborne chemicals emitted from the body has been found in many different pathologies and in particular for various forms of cancer. Metabolism of cancer cells is greatly altered during their lifetime; then, modification of chemicals is supposed to be large around cancer tissues. Positive hints in this direction were provided, as an example, on studying the breath composition of lung cancer-affected subjects. Besides the conventional analytical approaches, in recent years sensor arrays were also applied to these researches considering the chemical composition changes as those occurring in other applications such as for instance, those dealing with food quality measurements.Methods: In this paper, the first application of sensor arrays to study the differentiation between melanomas and nevi, namely malignant and benign affection of melanocytary cells, respectively, is presented and discussed. The localization of lesions on the skin surface made possible the utilization of differential measurements aimed at capturing the differences between two adjacent skin regions. This approach strongly reduces the influence of skin headspace variability due to the peculiar subjective odour background and the skin odour variability. The measurement campaign involved 40 cases; 10 of these were diagnosed melanomas referred to surgical intervention. Nine of these diagnoses were further confirmed by histological examinations of the removed tissue and one was a false positive.Results:The differences in the chemical composition of headspace were verified with a gas-chromatographic investigation, and the classification of electronic nose data provided an estimated cross-validated accuracy of the same order of magnitude as the currently used diagnostic instruments.Conclusion: Electronic nose sensors have been shown to have good sensitivity towards volatile organic compounds emitted by skin lesions, and the method seems to be effective for malign lesions identification. The results presented in this paper encourage a second experimental campaign with a larger number of participants and a systematic use of gas chromatography mass spectrometer technology in order to identify some possible melanoma biomarkers.