Characterization of a WRKY transcription factor expressed in late torpedo-stage embryos of Solanum chacoense

Characterization of a WRKY transcription factor expressed in late torpedo-stage embryos of Solanum chacoense
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DOI:
10.1007/s00425-004-1253-2
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发表时间:
2004-05-01
期刊:
影响因子:
4.3
通讯作者:
Matton, DP
Matton, DP
中科院分区:
生物学2区
文献类型:
--
作者:
Lagacé, M;Matton, DP

文献摘要

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从自交不亲和的野生马铃薯种Solanum chacoense Bitt胚珠中弱表达的mRNA筛选中分离出一种新的WRKY样转录因子。该蛋白被命名为ScWRKY1,由525个氨基酸组成,可以被分类为WRKY组I成员,具有两个WRKY结构域。它在茎、根和花瓣中以低水平表达,而在叶中以高得多的水平表达。有趣的是,虽然在发育中的种子中几乎检测不到,但它在受精胚珠中强烈且短暂地表达,这表明在胚胎发生过程中具有特定的作用。
A novel WRKY-like transcription factor was isolated from a screen for weakly expressed mRNAs in ovules in the self-incompatible wild potato species Solanum chacoense Bitt. This protein, named ScWRKY1, consisted of 525 amino acids and can be classified as a WRKY group-I member, having two WRKY domains. It is expressed at low levels in stems, roots, and petals, and expressed at much higher levels in leaves. Interestingly, although barely detectable in developing seeds, it is strongly and transiently expressed in fertilized ovules bearing late torpedo-staged embryos, suggesting a specific role during embryogenesis.