TRANSMITTER-LIKE ACTION OF ATP ON PATCHED MEMBRANES OF CULTURED MYOBLASTS AND MYOTUBES
TRANSMITTER-LIKE ACTION OF ATP ON PATCHED MEMBRANES OF CULTURED MYOBLASTS AND MYOTUBES
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DOI：
10.1038/303621a0
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发表时间：
1983-01-01
期刊：
影响因子：
64.8
通讯作者：
WAKELAM, MJO
中科院分区：
文献类型：
--
作者：
KOLB, HA;WAKELAM, MJO
由Burnstock 1首先提出的嘌呤能神经传递的概念已经在各种类型的细胞中得到证实。在这里，我们通过膜片钳方法2显示，在微电机浓度(1-100微米)的外部ATP激活融合能力成肌细胞和肌管的膜上的阳离子通道。在成肌细胞和肌管的细胞贴壁膜片上，外加ATP后，同时激活的通道的平均数目随着时间的延长而增加。在成肌细胞中，只有一组通道的平均单通道电导为γ=43ps，而在肌管中则有两组通道，γ1=48ps，γ2=20pS。用乙酰胆碱(ACh)或卡巴胆碱处理肌管膜可产生两组通道，它们的电导值和电压依赖的平均通道寿命与ATP反应产生的通道相似。结果表明，胚胎骨骼肌细胞含有对三磷酸腺苷敏感的阳离子通道，为三磷酸腺苷对这些细胞的神经递质样作用提供了证据。
The concept of purinergic neurotransmission, first proposed by Burnstock1, has been confirmed in various cell types. We show here, by the patch-clamp method2, that external ATP in micromotor concentrations (1–100 µM) activates cation channels in the membranes of fusion-competent myoblasts and myotubes. In cell-attached membrane patches of myoblasts and myotubes the mean number of simultaneously activated channels increases with time after external ATP application. In myoblasts only one population of channels having a mean single-channel conductance ofγ=43 pS was found, while in myotubes two populations withγ1=48 pS andγ2= 20 pS were observed. Treatment of myotube membranes with acetylcholine (ACh) or carbachol resulted in two populations of channels which had conductance values and voltage-dependent mean channel lifetimes similar to those produced in response to ATP. The results show that embryonic skeletal muscle cells contain cation channels sensitive to ATP and provide evidence for a neurotransmitter-like action of ATP on these cells.