HIV‐specific T lymphocyte immunity in mice immunized with a recombinant vaccinia virus
HIV‐specific T lymphocyte immunity in mice immunized with a recombinant vaccinia virus
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重组牛痘病毒免疫小鼠的 HIV 特异性 T 淋巴细胞免疫
DOI：
10.1002/eji.1830181208
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发表时间：
1988
影响因子：
5.4
通讯作者：
F. Plata
中科院分区：
文献类型：
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作者：
F. Michel;A. Hoffenbach;Pierre Lanlade‐Demoyen;B. Guy;M. Girard;J. Lecocq;S. Wain;M. Kieny;F. Plata
人类免疫缺陷病毒(HIV)感染可诱导人类、黑猩猩和猕猴的T细胞免疫。这种免疫反应的保护价值尚不清楚。因此，我们开发了一种小鼠实验系统，用于在体外和体内研究HIV特异性的CD4和CD8T淋巴细胞免疫。用表达HIV-1 gp160糖蛋白的痘苗病毒(VV)重组VV-11.39免疫BALB/c、DBA/2和C3H/He小鼠，用转HIV-1 env基因的组织相容靶细胞检测对HIV的原发和继发性细胞毒T淋巴细胞反应。杀伤细胞表达Thy-1和Ly-2(CD8)T细胞标志，受IH-2类组织相容性抗原限制。W-11.39免疫可明显诱导HIV-1包膜抗原的特异性免疫记忆：免疫前4周以上的小鼠脾细胞经HIV包膜抗原体外刺激后产生CD4和CD8 T淋巴细胞反应。这些反应的强度随着连续接种疫苗的增加而增加，表明艾滋病毒特异性前体T细胞池逐渐放大。最后，接种VV-11.39的DBA/2小鼠对表达HIV-1 env抗原的同种肿瘤产生了保护性免疫，导致加速了肿瘤排斥反应并提高了存活率。
Infection by the human immunodeficiency virus (HIV) induces T cell immunity in humans, chimpanzees and macaques. The protective value of this immune response is not clear. We have consequently developed a murine experimental system to study HIV‐specific CD4 and CD8 T lymphocyte immunity in vitro and in vivo. BALB/c, DBA/2 and C3H/He mice were immunized with vaccinia virus (VV) recombinant VV‐11.39 which expresses the gp160 glycoprotein of HIV‐1. Primary and secondary cytotoxic T lymphocyte response to HIV were detected with histocompatible mouse target cells transfected with the HIV‐1 env gene. Killer cells were positive for the Thy‐1 and Ly‐2 (CD8) T cell markers, and were restricted by class IH‐2 histocompatibility antigens. Immunological memory specific for HIV‐1 envelope antigens was clearly induced by vaccination with W‐11.39: spleen cells from mice vaccinated 4 weeks or more prior to assay generated CD4 and CD8 T lymphocyte responses following stimulation in vitro with HIV envelope antigens. The intensity of these responses increased with consecutive vaccinations, indicating that HIV‐specific precursor T cell pools were progressively amplified. Finally, DBA/2 mice vaccinated with VV‐11.39 developed protective immunity against a syngeneic tumor which expresses HIV‐1 env antigens, leading to accelerated tumor rejection and increased survival.
Morikawa,H;K.Sugino;Y.Hayashi;J.Takeda;M.Senda;A.Hirai;Y.Yamada：生物/技术。
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