Cholera Toxin Crystals Suitable for X-ray Diffraction
Cholera Toxin Crystals Suitable for X-ray Diffraction
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适用于 X 射线衍射的霍乱毒素晶体
DOI：
10.1126/science.197.4310.1277-a
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发表时间：
1977
期刊：
影响因子：
56.9
通讯作者：
R. Finkelstein
中科院分区：
文献类型：
--
作者：
P. Sigler;M. Druyan;H. Kiefer;R. Finkelstein
霍乱毒素的大晶体生长;它们的尺寸，对称性（P21），顺序和抗辐射性使它们非常适合高分辨率的X射线结构测定。每个不对称单位有一个分子（约84，000道尔顿），因此晶格没有显示分子对称性。两种不同的生物测定表明，来自溶解晶体的蛋白质保留了完整的生物活性。
Large crystals of the cholera toxin were grown; their dimensions, symmetry (P21), order, and resistance to radiation make them ideally suited for a high-resolution x-ray structure determination. There is one molecule (approximately 84,000 daltons) per asymmetric unit, and therefore the lattice reveals no molecular symmetry. Two distinct bioassays indicate that the protein from dissolved crystals retains full biological activity.