Nanoparticle Improved Stem Cell Therapy for Erectile Dysfunction in a Rat Model of Cavernous Nerve Injury
Nanoparticle Improved Stem Cell Therapy for Erectile Dysfunction in a Rat Model of Cavernous Nerve Injury
复制标题
DOI：
10.1016/j.juro.2015.10.129
复制
发表时间：
2016-03-01
影响因子：
6.6
通讯作者：
Wang, Run
中科院分区：
文献类型：
--
作者：
Lin, Haocheng;Dhanani, Nadeem;Wang, Run
目的：近年来，阴茎海绵体内注射干细胞作为治疗勃起功能障碍的一种潜在方法引起了人们极大的兴趣。然而，大多数干细胞在海绵体内注射后立即被冲洗掉。本研究的目的是调查使用NanoShuttle（TM）磁性纳米粒子，以保持干细胞在海绵体后，海绵体，从而提高勃起功能障碍的干细胞治疗在animal model.Materials和方法：脂肪来源的干细胞与NanoShuttle磁性纳米粒子磁化，以创建纳米脂肪来源的干细胞。将24只大鼠随机分为3组：脂肪干细胞组、纳米脂肪干细胞组和纳米脂肪干细胞+磁体组。在海绵体内注射后第1、3、5和9天追踪细胞。将40只双侧海绵体神经夹伤大鼠随机分为4组，分别为双侧海绵体神经夹伤组、双侧海绵体神经夹伤+海绵体内注射脂肪干细胞组、双侧海绵体神经夹伤+海绵体内注射纳米脂肪干细胞组和双侧海绵体神经夹伤+海绵体内注射纳米脂肪干细胞+磁铁组。结果：在体外实验中，1）NanoShuttle磁性纳米粒子成功地与脂肪干细胞结合，2）纳米脂肪干细胞向磁体迁移。在体内研究中，1）细胞追踪显示，使用磁体将纳米脂肪来源的干细胞成功地保留在海绵体中长达3天，而在海绵体内注射后第1天，在其他组中大多数脂肪来源的干细胞被洗出，和2）海绵体内压/平均动脉压，和aSMA（α-平滑肌肌动蛋白）和PECAM-1在纳米脂肪来源的干细胞组中，血小板内皮细胞粘附分子1的表达显著高于其他组。用NanoShuttle磁性纳米颗粒磁化脂肪来源的干细胞将脂肪来源的干细胞保持在海绵体中，并改善了动物模型中勃起功能障碍的脂肪来源的干细胞治疗。
Purpose: Recently intracavernous injection of stem cells has garnered great interest as a potential treatment of erectile dysfunction. However, most stem cells are washed out immediately after intracavernous injection. The goal of this study was to investigate using NanoShuttle (TM) magnetic nanoparticles to maintain stem cells in the corpus cavernosum after intracavernous injection, thereby improving stem cell therapy of erectile dysfunction in an animal model.Materials and Methods: Adipose derived stem cells were magnetized with NanoShuttle magnetic nanoparticles to create Nano-adipose derived stem cells. A total of 24 rats underwent bilateral cavernous nerve crush and were randomly assigned to 3 groups, including adipose derived stem cells, Nano-adipose derived stem cells and Nano-adipose derived stem cells plus magnet. Cells were tracked at days 1, 3, 5 and 9 after intracavernous injection. Another 40 rats with bilateral cavernous nerve crush were randomly assigned to 4 groups, including bilateral cavernous nerve crush, bilateral cavernous nerve crush plus adipose derived stem cell intracavernous injection, bilateral cavernous nerve crush plus Nanoadipose derived stem cell intracavernous injection and bilateral cavernous nerve crush plus Nano-adipose derived stem cell intracavernous injection plus magnet. Functional testing and histological analysis were performed 4 weeks after intracavernous injection.Results: In the in vitro study 1) NanoShuttle magnetic nanoparticles were successfully bound to adipose derived stem cells and 2) Nano-adipose derived stem cells migrated toward the magnet. In the in vivo study 1) cell tracking showed that Nano-adipose derived stem cells were successfully retained in the corpus cavernosum using the magnet for up to 3 days while most adipose derived stem cells were washed out in other groups by day 1 after intracavernous injection, and 2) intracavernous pressure/mean arterial pressure, and aSMA (a-smooth muscle actin) and PECAM-1 (platelet endothelial cell adhesion molecule 1) expression in the Nano-adipose derived stem cell group was significantly higher than in the other groups.Conclusions: Magnetization of adipose derived stem cells with NanoShuttle magnetic nanoparticles kept adipose derived stem cells in the corpus cavernosum and improved adipose derived stem cell therapy of erectile dysfunction in an animal model.