Calcitonin gene-related peptide and nitric oxide in the trigeminal ganglion: Cerebral vasodilatation from trigeminal nerve stimulation involves mainly calcitonin gene-related peptide
Calcitonin gene-related peptide and nitric oxide in the trigeminal ganglion: Cerebral vasodilatation from trigeminal nerve stimulation involves mainly calcitonin gene-related peptide
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DOI：
10.1016/s0165-1838(98)00033-2
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发表时间：
1998-05-28
期刊：
影响因子：
--
通讯作者：
Uddman, R
中科院分区：
文献类型：
--
作者：
Edvinsson, L;Mulder, H;Uddman, R
一氧化氮(NO)是一种新的神经递质候选物质，被认为具有多种生理功能。在本研究中，用免疫细胞化学的方法证明了一氧化氮合酶(NOS)的存在，并研究了它与其他神经递质的可能共存。猫三叉神经节内可见中等数量的一氧化氮合酶免疫反应神经元胞体，其中大部分还表达降钙素基因相关肽。三叉神经节内表达一氧化氮合酶的神经细胞体以中小型为主，胞体数量众多，大小不一。用寡核苷酸探针进行原位杂交，在猫的三叉神经细胞中几乎所有的神经元都有corp基因的表达。刺激鼻睫神经导致同侧局部皮质血流量频率依赖性增加30+/-6%。静脉或皮质表面应用一氧化氮合酶抑制剂N-G-硝基-L-精氨酸甲酯(L-NAME)对此反应无明显影响。局部皮质注射CGRP阻滞剂h-CGRP(8-37)并不改变对高碳酸血症或静息血流的脑血管扩张剂反应。但经h-CGRP(8-37)处理后，鼻睫神经反应减少50%，频率-反应曲线总体右移。这些结果表明，尽管一氧化氮合酶存在于三叉神经节细胞中，并与降钙素基因相关肽共存于部分感觉神经元，但其在三叉神经介导的血管扩张中的作用并不显著。(C)1998 Elsevier Science B.V.保留所有权利。
Nitric oxide (NO) is a novel neurotransmitter candidate to which a large number of physiological roles has been ascribed. In the present study, immunocytochemistry was used to demonstrate NO synthase (NOS) and to investigate possible co-localization with other neurotransmitters. In the trigeminal ganglion of the cat, a moderate number of NOS immunoreactive nerve cell bodies was seen, of which the major part also expressed calcitonin gene-related peptide (CGRP). The nerve cell bodies expressing NOS in the trigeminal ganglion were predominantly of small to medium size; while numerous cell bodies of varying size contained CORP. With in situ hybridization using oligonucleotide probes, CORP mRNA was demonstrated in almost all trigeminal neurons of the cat. Stimulation of the nasociliary nerve resulted in a frequency-dependent increase in ipsilateral local cortical blood flow by 30 +/- 6%. Administration of the NOS inhibitor N-G-nitro-L-arginine-methylester (L-NAME) did not significantly alter this response when applied intravenously or on the cortical surface. Local cortical administration of the CGRP blocker h-CGRP (8-37) did not alter the cerebral vasodilator response to hypercapnia or resting flow. However, the nasociliary nerve response was reduced by 50% after h-CGRP (8-37), with a general shift to the right of the frequency-response curve. These data suggest that although NOS is seen in several trigeminal ganglion cells and coexists with CGRP in a subpopulation of the sensory neurons, its role in trigeminally mediated vasodilatation was not significant. (C) 1998 Elsevier Science B.V. All rights reserved.