A single-molecule nanopore device detects DNA polymerase activity with single-nucleotide resolution
A single-molecule nanopore device detects DNA polymerase activity with single-nucleotide resolution
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DOI：
10.1021/ja077082c
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发表时间：
2008-01-23
影响因子：
15
通讯作者：
Ghadiri, M. Reza
中科院分区：
文献类型：
--
作者：
Cockroft, Scott L.;Chu, John;Ghadiri, M. Reza
以单核苷酸分辨率监测DNA聚合酶活性的能力已经成为许多先进的单分子DNA测序概念的基石。为了实现这一目标，我们报告了在单分子水平上首次观察到的单碱基分辨率的碱基-碱基DNA聚合酶活性。我们描述了一种超分子纳米孔装置的设计和表征，该装置能够以高灵敏度和空间分辨率检测多达9个连续的DNA聚合酶催化的单核苷酸引物延伸（
The ability to monitor DNA polymerase activity with single-nucleotide resolution has been the cornerstone of a number of advanced single-molecule DNA sequencing concepts. Toward this goal, we report the first observation of the base-by-base DNA polymerase activity with single-base resolution at the single-molecule level. We describe the design and characterization of a supramolecular nanopore device capable of detecting up to nine consecutive DNA polymerase-catalyzed single-nucleotide primer extensions with high sensitivity and spatial resolution (