ULTRASTRUCTURE OF LEYDIG CELLS AS REVEALED BY SECONDARY TISSUE TREATMENT WITH A FERROCYANIDE-OSMIUM MIXTURE
ULTRASTRUCTURE OF LEYDIG CELLS AS REVEALED BY SECONDARY TISSUE TREATMENT WITH A FERROCYANIDE-OSMIUM MIXTURE
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DOI：
10.1016/0040-8166(77)90040-4
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发表时间：
1977-01-01
期刊：
影响因子：
2.6
通讯作者：
BURGUET, S
中科院分区：
文献类型：
--
作者：
RUSSELL, L;BURGUET, S
常规制备的间质细胞(戊二醛-Os)用于超微结构研究，由于细胞膜保存不良和细胞质基质致密，通常发现缺乏亚细胞细节。在Karnovsky(1971)的方法之后进行了改进，利用亚铁氰化铯混合物对戊二醛固定的组织进行后处理，发现常规方法可以很好地保存间质细胞。这种方法的主要优点是增强了间质细胞内部的对比度，并大大改善了膜的保存。此外，光滑的内质网通常表现为直径均匀的相互连接的小管组成的广泛网络，尤其是线粒体、溶酶体、过氧体、多囊泡体和高尔基体。在这些细胞中发现了大量的糖原和微丝，这在常规制备中很难看到。对亚细胞器的数量和分布进行了新的观察，并讨论了它们在类固醇合成过程中的可能作用。鉴于本研究中观察到的组织保存效果显著改善，该处理可能被常规用于保存大鼠间质细胞。
Leydig cells prepared routinely (glutaraldehyde-osmium) for ultrastructural studies are generally found to be lacking in subcellular detail as a result of poor membrane preservation and a dense cytoplasmic matrix. A method modified after that of Karnovsky (1971), utilizing a ferrocyanide osmium mixture for post-treating glutaraldehyde fixed tissues, was found to yield routinely excellent preservation of Leydig cells. The primary advantages of this method were the enhancement of contrast within the Leydig cell and greatly improved membrane preservation. In addition, the smooth endoplasmic reticulum always appeared as an extensive network of interconnected tubules of uniform diameter; mitochondria, lysosomes, peroxisomes, multivesicular bodies and Golgi were especially prominent. Glycogen and microfilaments, not readily seen in routine preparations, were found to be abundant in these cells. New observations on the numbers and distributions of subcellular organelles are described and are discussed in relation to their possible role in the steroidogenic process. In view of the greatly improved tissue preservation observed in this study, this treatment may be used routinely for preservation of rat Leydig cells.