Cytotoxicity, antiviral and antimicrobial activities of alkaloids, flavonoids, and phenolic acids
Cytotoxicity, antiviral and antimicrobial activities of alkaloids, flavonoids, and phenolic acids
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DOI：
10.3109/13880209.2010.519390
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发表时间：
2011-04-01
影响因子：
3.8
通讯作者：
Orhan, Ilkay
中科院分区：
文献类型：
--
作者：
Ozcelik, Berrin;Kartal, Murat;Orhan, Ilkay
材料和方法：测试化合物对DNA病毒单纯疱疹1型和RNA病毒副流感（3型）的抗病毒活性。使用Madin-Darby牛肾和Vero细胞系测定化合物的细胞毒性，并将其致细胞病变效应表示为最大无毒浓度。对以下细菌及其分离菌株进行了抗菌活性测定：大肠杆菌、铜绿假单胞菌、奇异变形杆菌、肺炎克雷伯菌、鲍曼不动杆菌、金黄色葡萄球菌、粪肠球菌和枯草芽孢杆菌，尽管它们是通过微量稀释法对两种真菌进行筛选的：白色念珠菌和近平滑念珠菌。阿托品和没食子酸在0.8--0.05 μ A μ g/ml范围内有较强的抗病毒作用
Materials and methods: Antiviral activity of the compounds was tested against DNA virus herpes simplex type 1 and RNA virus parainfluenza (type-3). Cytotoxicity of the compounds was determined using Madin-Darby bovine kidney and Vero cell lines, and their cytopathogenic effects were expressed as maximum non-toxic concentration. Antibacterial activity was assayed against following bacteria and their isolated strains: Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis, Klebsiella pneumoniae, Acinetobacter baumannii, Staphylococcus aureus, Enterococcus faecalis, and Bacillus subtilis, although they were screened by microdilution method against two fungi: Candida albicans and Candida parapsilosis.Results: Atropine and gallic acid showed potent antiviral effect at the therapeutic range of 0.8--0.05 mu A mu g ml