Real-Time Testing of Bioburdens in Metalworking Fluids Using Adenosine Triphosphate as a Biomass Indicator
Real-Time Testing of Bioburdens in Metalworking Fluids Using Adenosine Triphosphate as a Biomass Indicator
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使用三磷酸腺苷作为生物量指示剂实时测试金属加工液中的生物负载
DOI：
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发表时间：
2009
期刊：
影响因子：
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通讯作者：
M. Deschepper
中科院分区：
文献类型：
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作者：
F. Passman;G. Egger;Sarah Hallahan;Brent W. Skinner;M. Deschepper
自20世纪50年代初以来，三磷酸腺苷（ATP）测定已被用于定量低有机化合物含量流体（淡水、海水、冷却塔水和类似流体）中的生物负荷（生物量）。最初的方法是劳动密集型的，需要相当的实验室技能。在过去的半个世纪里，该方案已经大大简化，但直到最近，化学干扰使得在金属加工液（MWF）中使用ATP测试变得不切实际。本文介绍了一种新的乳化油、半合成和合成MWF中最终使用稀释度的ATP测试方案的精度和偏差统计。此外，还介绍了现场试验的结果，其中ATP数据与其他MWF状态监测数据进行了比较。现场评价结果表明，新方案适用于MWF生物负荷状态监测。
Adenosine triphosphate (ATP) assays have been used to quantify bioburdens (biomass) in low-organic-compound-content fluids (freshwater, seawater, cooling tower water, and similar fluids) since the early 1950s. The original methodology was labor intensive and required considerable laboratory skill. Over the past half-century, the protocol has been simplified substantially, but until recently, chemical interferences made it impractical to use the ATP test in metalworking fluids (MWF). This article presents precision and bias statistics for a new test protocol for ATP in emulsifiable oil, semisynthetic and synthetic MWF at end-use dilutions. Additionally it presents the results of field tests in which ATP data are compared with other MWF condition monitoring data. The field evaluation demonstrates the applicability of the new protocol to MWF bioburden condition monitoring.