Ligand assays: from electrophoresis to miniaturized microarrays.
Ligand assays: from electrophoresis to miniaturized microarrays.
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DOI：
10.1093/clinchem/44.9.2015
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发表时间：
1998-09
影响因子：
9.3
通讯作者：
R. Ekins
中科院分区：
文献类型：
--
作者：
R. Ekins
对我所参与的“配基分析”领域的主要进展进行了追踪。其中包括依靠放射性标记分析标记物的“第一代”竞争性分析方法的原创性开发；第一代“第二代”非竞争性(超灵敏)方法的开发，其依赖于标记(单抗)抗体和高比活性非同位素标记的使用(导致20世纪80年代免疫诊断领域的变革)；以及第一种“第三代”小型化芯片微阵列方法的开发，这些方法允许同时对同一小样本中的许多分析物进行超灵敏测量。后者--既适用于免疫分析，也适用于DNA/RNA分析--很可能在未来十年内给诊断和制药领域带来革命性的变化。
The main developments in the "ligand assay" field in which I have been involved are traced. These include the original development of "first generation" competitive assays relying on radiolabeled analyte markers; the development of the first "second generation", noncompetitive (ultrasensitive) methods, which rely on the use of labeled (monoclonal) antibodies and high specific activity nonisotopic labels (leading to the transformation of the immunodiagnostic field in the 1980s); and the development of the first "third generation" miniaturized, chip-based, microarray methods, which permit the simultaneous ultrasensitive measurement of many analytes in the same small sample. The latter--applicable both to immunoassay and to DNA/RNA analysis--are likely to revolutionize the diagnostic and pharmaceutical fields in the next decade.