Selenium alleviates chromium toxicity by preventing oxidative stress in cabbage (Brassica campestris L. ssp. Pekinensis) leaves.
Selenium alleviates chromium toxicity by preventing oxidative stress in cabbage (Brassica campestris L. ssp. Pekinensis) leaves.
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DOI：
10.1016/j.ecoenv.2015.01.026
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发表时间：
2015-04
影响因子：
6.8
通讯作者：
Xuejiao Qing;Xiaohu Zhao;Chengxiao Hu;Peng Wang;Ying Zhang;Xuan Zhang;Pengcheng Wang;Hanzhi Shi-Hanzhi
中科院分区：
文献类型：
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作者：
Xuejiao Qing;Xiaohu Zhao;Chengxiao Hu;Peng Wang;Ying Zhang;Xuan Zhang;Pengcheng Wang;Hanzhi Shi-Hanzhi
硒(Se)在减轻铬(Cr)诱导的氧化应激中的有益作用已得到充分证实。然而，人们对其潜在机制知之甚少。用细胞和生化方法研究了外源Se(0.1 mg/L)对铬(1 mg/L)诱导的北京甘蓝(Brassica campestris L.ssp.Pekinensis)叶片氧化胁迫和抗氧化系统的影响。结果表明，添加Se能有效降低铬诱导的过氧化脂质和超氧阴离子自由基(O-−-2·)水平，提高超氧化物歧化酶和过氧化物酶活性。同时，1 mg/L铬引起的质膜完整性丧失、生长抑制以及叶片超微结构的变化均显著逆转。此外，施Se显著改变了铬从线粒体、细胞核和细胞壁物质向可溶性部分和叶绿体转运的亚细胞分布。而施Se对铬对渗透调节积累产物的影响不显著。研究表明，Se能够减轻铬诱导的氧化胁迫，并使铬重新分布在叶片的亚细胞中，从而保护甘蓝叶片免受铬毒害。此外，自由基、过氧化脂质、超氧化物歧化酶和过氧化物酶活性以及铬的亚细胞分布可被认为是表明硒对铬的解毒效果的有效生物标志物。
The beneficial role of selenium (Se) in alleviation of chromium (Cr)-induced oxidative stress is well established. However, little is known about the underlying mechanism. The impacts of exogenous Se (0.1 mg/L) on Cr(1 mg/L)-induced oxidative stress and antioxidant systems in leaves of cabbage (Brassica campestris L. ssp.Pekinensis) were investigated by using cellular and biochemical approaches. The results showed that supplementation of the medium with Se was effective in reducing Cr-induced increased levels of lipid peroxides and superoxide free radicals (O−2·), as well as increasing activities of superoxide dismutase (SOD) and peroxidase (POD). Meanwhile, 1 mg/L Cr induced loss of plasma membrane integrity, growth inhibition, as well as ultrastructural changes of leaves were significantly reversed due to Se supplementation in the medium. In addition, Se application significantly altered the subcellular distribution of Cr which transported from mitochondria, nucleus and the cell-wall material to the soluble fraction and chloroplasts. However, Se application did no significant alteration of Cr effects on osmotic adjustment accumulating products. The study suggested that Se is able to protect leaves of cabbage against Cr toxicity by alleviation of Cr induced oxidative stress, and re-distribution of Cr in the subcellular of the leaf. Furthermore, free radicals, lipid peroxides, activity of SOD and POD, and subcellular distribution of Cr can be considered the efficient biomarkers to indicate the efficiency of Se to detoxification Cr.