Cellular localization of intestinal calcium-binding protein in pig duodenum.
Cellular localization of intestinal calcium-binding protein in pig duodenum.
复制标题
猪十二指肠肠钙结合蛋白的细胞定位。
作者：
B. M. Arnold;K. Kovács;T. Murray
采用免疫过氧化物酶法，利用兔抗猪肠钙结合蛋白（CaBP）血清γ -球蛋白部分研究CaBP在猪十二指肠组织中的定位。十二指肠上皮细胞的细胞质内可见特异性免疫染色，表明CaBP的存在。杯状细胞及上皮下层未检出CaBP。当特异性抗体被非免疫兔γ -球蛋白替代或特异性抗体被过量的CaBP预吸附时，未见阳性免疫染色。我们的研究支持CaBP可能在细胞内钙运输中起作用的假设。
The gamma-globulin fraction of a rabbit antiserum against porcine intestinal calcium-binding protein (CaBP) was used in an immunoperoxidase method to study CaBP localization in porcine duodenal tissue. Specific immunostaining, indicative of the presence of CaBP, was noted within the cytoplasm of the duodenal epithelial cells. No CaBP was detected in goblet cells or in the subepithelial layers. When the specific antibody was replaced by either nonimmune rabbit gamma-globulin or when the specific antibody was preadsorbed with excess CaBP, no positive immunostaining was seen. Our studies lend support to the hypothesis that CaBP may function in intracellular calcium transport.