Confocal microscopy with strip mosaicing for rapid imaging over large areas of excised tissue
Confocal microscopy with strip mosaicing for rapid imaging over large areas of excised tissue
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DOI：
10.1117/1.jbo.18.6.061227
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发表时间：
2013-06-01
影响因子：
3.5
通讯作者：
Rajadhyaksha, Milind
中科院分区：
文献类型：
--
作者：
Abeytunge, Sanjee;Li, Yongbiao;Rajadhyaksha, Milind
共焦镶嵌显微镜是一种正在开发的技术平台，可直接在新鲜切除的组织中对肿瘤边缘进行成像，无需传统病理学所需的处理。此前，在 9 分钟内即可对莫氏手术切除的 12-x -12 mm(2) 皮肤组织进行镶嵌并检测基底细胞癌边缘。去年，我们报告了一种称为“条带镶嵌”的更快方法的可行性，该方法在 3 分钟内在 10- x -10 mm(2) 组织上进行了演示。在这里，我们描述了仪器、软件和速度方面的进一步进步。还开发了一种使组织变平的机制，以便能够在大面积上一致且可重复地采集图像。我们展示了在 90 秒内以 1-μm 横向分辨率在 10- x -10 mm(2) 皮肤组织上进行镶嵌。在 13 分钟内以 0.8 μm 横向分辨率扫描一块 2.5- x -3.5 cm(2) 的乳腺组织。在大面积新鲜组织上快速拼接共焦图像可能提供一种在床边进行病理学的方法。使用条带镶嵌共聚焦显微镜对肿瘤边缘进行成像可以作为传统（冷冻或固定）病理学的辅助手段来指导手术。 (C) 作者。由 SPIE 根据 Creative Commons Attribution 3.0 Unported 许可证发布。全部或部分分发或复制本作品需要原始出版物的完整归属，包括其 DOI。
Confocal mosaicing microscopy is a developing technology platform for imaging tumor margins directly in freshly excised tissue, without the processing required for conventional pathology. Previously, mosaicing on 12-x -12 mm(2) of excised skin tissue from Mohs surgery and detection of basal cell carcinoma margins was demonstrated in 9 min. Last year, we reported the feasibility of a faster approach called "strip mosaicing," which was demonstrated on a 10- x -10 mm(2) of tissue in 3 min. Here we describe further advances in instrumentation, software, and speed. A mechanism was also developed to flatten tissue in order to enable consistent and repeatable acquisition of images over large areas. We demonstrate mosaicing on 10- x -10 mm(2) of skin tissue with 1-mu m lateral resolution in 90 s. A 2.5- x -3.5 cm(2) piece of breast tissue was scanned with 0.8-mu m lateral resolution in 13 min. Rapid mosaicing of confocal images on large areas of fresh tissue potentially offers a means to perform pathology at the bedside. Imaging of tumor margins with strip mosaicing confocal microscopy may serve as an adjunct to conventional (frozen or fixed) pathology for guiding surgery. (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.