The membrane chamber: A new type of in vitro recording chamber
The membrane chamber: A new type of in vitro recording chamber
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DOI：
10.1016/j.jneumeth.2010.10.024
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发表时间：
2011-01-30
影响因子：
3
通讯作者：
Greenfield, S. A.
中科院分区：
文献类型：
--
作者：
Hill, M. R. H.;Greenfield, S. A.
体外脑切片电生理学是一项强大且非常成功的技术，从大脑上切下薄片，并在记录室中人工保存。该记录室的设计对此类实验的成功和质量至关重要。目前通常使用两种类型的腔室，界面腔室或水下腔室。然而，这些腔室的缺点是它们分别在实验或生理特性上受到限制。本文提出了一种新的体外腔设计原理，旨在结合经典设计的优点，同时克服它们的缺点。这是通过使用放置切片的半透膜来实现的。该膜允许人工脑脊液快速流动，流速至少为17ml /min。由于伯努利效应，这种高速流动也会导致溶液穿过薄片所在膜的流量增加64%。由于脑膜是透明的，因此为脑电生理切片提供了宽视场倒置光学成像的可能性。在小于1/8 mm(2)的区域同时记录局部场电位、单细胞和电压敏感染料成像数据，证明了这种设置的实用性。膜腔中所有这些特征的结合使其成为许多当前和未来体外应用的多功能和有前途的设备，特别是在光学成像方面。(C) 2010 Elsevier B.V.版权所有
In vitro brain slice electrophysiology is a powerful and highly successful technique where a thin slice is cut from the brain and kept alive artificially in a recording chamber. The design of this recording chamber is pivotal to the success and the quality of such experiments. Most often one of two types of chambers is used today, the interface chamber or the submerged chamber. These chambers, however, have the disadvantage that they are limited in either their experimental or their physiological properties respectively. Here we present a new working principle for an in vitro chamber design which aims at combining the advantages of the classical designs whilst overcoming their disadvantages. This is achieved by using a semipermeable membrane on which the slice is placed. The membrane allows for a fast flow of artificial cerebrospinal fluid of up to at least 17 ml/min. Due to a Bernoulli effect, this high speed flow also causes a 64% increase in flow of solution across the membrane on which the slice rests. The fact that the membrane is transparent introduces the possibility of wide field inverted optical imaging to brain slice electrophysiology. The utility of this setup was demonstrated in the recording of local field potential, single cell and voltage sensitive dye imaging data simultaneously from an area smaller then 1/8 mm(2). The combination of all these features in the membrane chamber make it a versatile and promising device for many current and future in vitro applications, especially in the regard to optical imaging. (C) 2010 Elsevier B.V. All rights reserved.