EFFECTS OF CIPROFLOXACIN, NORFLOXACIN, AND OFLOXACIN ON IN-VITRO ADHESION AND SURVIVAL OF PSEUDOMONAS-AERUGINOSA AK1 ON URINARY CATHETERS
EFFECTS OF CIPROFLOXACIN, NORFLOXACIN, AND OFLOXACIN ON IN-VITRO ADHESION AND SURVIVAL OF PSEUDOMONAS-AERUGINOSA AK1 ON URINARY CATHETERS
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DOI：
10.1128/aac.38.7.1490
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发表时间：
1994-07-01
影响因子：
4.9
通讯作者：
BRUCE, AW
中科院分区：
文献类型：
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作者：
REID, G;SHARMA, S;BRUCE, AW
在体外用0.1和0.5 μ g/ml环丙沙星预处理尿硅胶乳胶导管1、24和48 h，可显著降低临床分离株铜绿假单胞菌AK 1的粘附和存活。紫外光谱和高效液相色谱证实了吸附在导管上的环丙沙星的存在，并显示高达56%的药物在24小时内浸出到周围液体中。扫描电子显微镜显示，粘附微生物变形和延长暴露于环丙沙星后。透射电子显微镜显示菌毛存在于细菌表面，但没有确凿的证据表明菌毛暴露于环丙沙星后发生变化。结果发现，一个显着的根除24小时假单胞菌生物膜可以实现与环丙沙星以及与氧氟沙星和诺氟沙星。与对照导管相比，用50-和100-μ g/ml浓度的环丙沙星预孵育导管导致粘附细菌数量减少高达99%。此外，粘附的生物膜通过在pH 7.0和5.5下用50和100 μ g/ml环丙沙星攻击24小时而被根除。这些体外研究的结果表明，氟喹诺酮类药物在预防和治疗与铜绿假单胞菌粘附假体器械相关的尿路感染方面可能具有临床作用。
Pretreatment of urinary silicone latex catheters in vitro with 0.1 and 0.5 mu g of ciprofloxacin per ml for 1, 24, and 48 h significantly reduced the adhesion and survival of the clinical isolate Pseudomonas aeruginosa AK1. W spectroscopy and high-performance liquid chromatography confirmed the presence of ciprofloxacin adsorbed onto the catheters and showed that up to 56% of the drug Leached into the surrounding fluid within 24 h. Scanning electron microscopy demonstrated that the adherent organisms were malformed and elongated after exposure to ciprofloxacin. Transmission electron microscopy showed the presence of fimbriae on the bacterial surfaces, but there was no conclusive evidence of changes in the fimbriae upon exposure to ciprofloxacin. It was found that a significant eradication of 24-h Pseudomonas biofilms could be achieved with ciprofloxacin as well as with ofloxacin and norfloxacin. Preincubation of catheters with 50- and 100-mu g/ml concentrations of ciprofloxacin resulted in up to a 99% reduction in the number of adherent bacteria in comparison with the reduction on control catheters. In addition, adherent biofilms were eradicated by 24 h of challenge with 50 and 100 mu g of ciprofloxacin per ml at pH 7.0 and 5.5. Results of these in vitro studies suggest that there could be a clinical role for fluoroquinolones in preventing and treating urinary tract infections associated with P. aeruginosa adherence to prosthetic devices.