Biosynthesis of cerium oxide nanoparticles and their effect on lipopolysaccharide (LPS) induced sepsis mortality and associated hepatic dysfunction in male Sprague Dawley rats
Biosynthesis of cerium oxide nanoparticles and their effect on lipopolysaccharide (LPS) induced sepsis mortality and associated hepatic dysfunction in male Sprague Dawley rats
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DOI：
10.1016/j.msec.2017.11.014
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发表时间：
2018-02-01
影响因子：
7.9
通讯作者：
Xu, Yunfei
中科院分区：
文献类型：
--
作者：
Chen, Guoqiang;Xu, Yunfei
败血症是一种发展为“血液中毒”的紧急医疗灾难。脓毒症是导致患者死亡的器官疾病之一。模拟脓毒症化学变化的研究模型是必要的。我们已经合成了CeO 2纳米粒子使用环保的方法，使用植物提取物。采用UV-Vis、FTIR、XRD、SEM、EDX和TEM等分析手段对合成的CeO 2纳米粒子进行了表征。结果表明，合成的CeO 2为纳米级球形。对脂多糖（LPS）雄性Sprague道利大鼠评价合成的CeO 2纳米颗粒。用CeO_2纳米粒（0.5mg/Ag）静脉注射感染SD大鼠，观察其死亡率、呼吸频率、血压、体温等指标。大鼠可恢复所有参数，肝损伤、血清细胞因子/趋化因子和肿胀指标降低。用CeO 2 NPs处理的枯否细胞和巨噬细胞显示出最低的TNF-α、IL-1b、IL-6和HMGB 1释放。由于ROS、iNOS、考克斯-2和核因子-κ轻链的存在，触发的B细胞（NF-κ B）转录作用降低。因此，结果表明CeO 2纳米颗粒可用作肝脓毒症的愈合剂。
The fetal/life challenging Sepsis is an urgent medical disaster which have developed "blood poisoning". The sepsis is one of the disorders of organs which cause death in patients. The investigational models that mimic-chemical change of sepsis in human bodywereessential. We have synthesized CeO2 NPs using eco-friendly method using plant extract. The characterization of synthesized CeO2 NPs was studied using UV-Vis, FTIR, XRD, SEM, EDX and TEM analysis. The results were confirmed that the synthesized CeO2 was in nano size with spherical shape. The synthesized CeO2 nanoparticles were evaluated against lipopolysaccharide (LPS) male Sprague Dawley rats. We have intravenously administrated CeO2 NPs (0.5 mg/Ag) to Sprague Dawleyratswhich are septic and checked the death rate, respiratory rate, blood pressure, body temperature. The rats could restore all parameters with decrease in hepatic damage, serum cytokines/chemokines, and swelling indicators. The Kupffer cells and macrophage cells treated with CeO2 NPs and showed lowest TNF-alpha, IL-1b, IL-6, and HMGB1release. Due to the ROS, iNOS, COX-2, and nuclear factor-kappa light chain, the triggered B cells (NF-kB) transcriptional action was decreased. Thus, results represents that CeO2 NPs can be applied as healing agent for liver sepsis.