Botryorhodines A-D, antifungal and cytotoxic depsidones from Botryosphaeria rhodina, an endophyte of the medicinal plant Bidens pilosa
Botryorhodines A-D, antifungal and cytotoxic depsidones from Botryosphaeria rhodina, an endophyte of the medicinal plant Bidens pilosa
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DOI：
10.1016/j.phytochem.2009.09.024
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发表时间：
2010-01-01
期刊：
影响因子：
3.8
通讯作者：
Hertweck, Christian
中科院分区：
文献类型：
--
作者：
Abdou, Randa;Scherlach, Kirstin;Hertweck, Christian
从菊科药用植物三叶鬼针草（Bidens pilosa）的茎中分离到一种内生真菌（Botryosphaeria rhodina），该真菌具有抗炎、防腐和抗真菌作用。真菌分离物的乙酸乙酯提取物对几种癌细胞系表现出显著的抗真菌活性以及有效的细胞毒性和抗增殖作用。活性导向分馏导致分离四个depropathones，botryorhodines A-D和生长素吲哚羧酸的复合物。Botryorhodine A和B对HeLa细胞系显示中等至弱的细胞毒活性，其CC_（50）分别为96.97 μ M和36.41 μ M。此外，它们还显示出抗一系列病原真菌的抗真菌活性，例如土曲霉（对葡萄红菌素A的MIC为26.03 μ M，对B的MIC为49.70 μ M）和植物病原体尖孢镰刀菌（对葡萄红菌素A的MIC为191.60 μ M，对B的MIC为238.80 μ M）。内生菌在调节真菌种群内生活或攻击宿主植物的潜在作用进行了讨论。(C)2009爱思唯尔有限公司保留所有权利。
An endophytic fungus (Botryosphaeria rhodina) was isolated from the stems of the medicinal plant Bidens pilosa (Asteraceae) that is known for its anti-inflammatory, antiseptic and antifungal effects. The ethyl acetate extract of the fungal isolate exhibits significant antifungal activity as well as potent cytotoxic and antiproliferative effects against several cancer cell lines. Activity-guided fractionation resulted in the isolation of a complex of four depsidones, botryorhodines A-D and the auxin indole carboxylic acid. Botryorhodine A and B show moderate to weak cytotoxic activities against HeLa cell lines with a CC50 Of 96.97 mu M and 36.41 mu M, respectively. In addition, they also show antifungal activity against a range of pathogenic fungi such as Aspergillus terreus (MIC 26.03 mu M for botryorhodine A and 49.70 mu M for B) and the plant pathogen Fusarium oxysporum (MIC 191.60 mu M for botryorhodine A and 238.80 mu M for B). A potential role of the endophyte in modulating fungal populations living within or attacking the host plant is discussed. (C) 2009 Elsevier Ltd. All rights reserved.