Lenslet array tunable snapshot imaging spectrometer (LATIS) for hyperspectral fluorescence microscopy
Lenslet array tunable snapshot imaging spectrometer (LATIS) for hyperspectral fluorescence microscopy
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DOI：
10.1364/boe.8.001950
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发表时间：
2017-03-01
影响因子：
3.4
通讯作者：
Tkaczyk, Tomasz S.
中科院分区：
文献类型：
--
作者：
Dwight, Jason G.;Tkaczyk, Tomasz S.
快照高光谱成像增强了现有扫描系统的像素驻留时间和采集速度，使其成为荧光显微镜的强大工具。虽然大多数快照系统包含固定的数据集参数（x, y,lambda），但我们的新型快照系统，称为透镜阵列可调快照成像光谱仪（LATIS），展示了在485至660 nm范围内将其平均光谱分辨率从22.66 nm （80x80x22）调整到13.94 nm （88x88x46）。我们还描述了一个具有200x200x27数据立方体的固定LATIS，用于更大的视场（FOV）成像。我们报告了< 1秒曝光时间和高分辨率荧光成像与最小的伪影。(C) 2017年美国光学学会
Snapshot hyperspectral imaging augments pixel dwell time and acquisition speeds over existing scanning systems, making it a powerful tool for fluorescence microscopy. While most snapshot systems contain fixed datacube parameters (x, y,lambda), our novel snapshot system, called the lenslet array tunable snapshot imaging spectrometer (LATIS), demonstrates tuning its average spectral resolution from 22.66 nm (80x80x22) to 13.94 nm (88x88x46) over 485 to 660 nm. We also describe a fixed LATIS with a datacube of 200x200x27 for larger field-of-view (FOV) imaging. We report < 1 sec exposure times and high resolution fluorescence imaging with minimal artifacts. (C) 2017 Optical Society of America