Endothelin releasing activity in calf serum and porcine follicular fluid.
Endothelin releasing activity in calf serum and porcine follicular fluid.
复制标题
小牛血清和猪卵泡液中内皮素释放活性。
DOI：
10.1016/s0006-291x(05)80859-9
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发表时间：
1990
影响因子：
3.1
通讯作者：
Vale,W
中科院分区：
文献类型：
--
作者：
Brown,MR;Vaughan,J;Walsh,J;Jimenez,L;Hexum,TD;Baird,A;Vale,W
牛血清（CS）和猪卵泡液（PFF）加入培养的牛主动脉内皮细胞（BAEC）刺激内皮素（ET）的释放。使用Bond Elut C18对酸化小牛血清和PFF进行吸附剂萃取，得到活性为起始材料活性30%的产物。使用HPLC对Bond Elut C18提取的CS或PFF进行分级，得到保留时间分别与TGFβ和激活素A相似的活性峰。TGFβ刺激BAEC释放ET的能力是激活素A的10倍。TGFβ和激活素A刺激ET释放的剂量相加而非效应相加的观察结果与这两种物质通过类似机制起作用的结论一致。因此，TGFβ和/或激活素A相关肽可能有助于在酸化Bond Elut C18提取的CS和PFF中观察到的ET释放活性。天然CS和PFF中ET释放活性的同一性仍有待确定。
Bovine calf serum (CS) and porcine follicular fluid (PFF) added to bovine aortic endothelial cells (BAEC) in culture stimulate endothelin (ET) release. Sorbent extraction of acidified calf serum and PFF using Bond Elut C18yields a product with an activity 30% that of the starting material. Fractionation of Bond Elut C18extracted CS or PFF using HPLC yields peaks of activity with retention times similar to those of TGFβand activin A, respectively. TGFβis 10-fold more potent than activin A to stimulate ET release from BAECs. The observation that TGFβand activin A are dose-additive but not effect-additive to stimulate ET release is compatible with the conclusion that these two substances act through a similar mechanism. Thus, TGFβand/or activin A-related peptides may contribute to the ET releasing activity observed in acidified Bond Elut C18extracted CS and PFF. The identity of ET-releasing activity in native CS and PFF remains to be established.