Intestinal Adaptation Following Massive Ileocecal Resection in 20-day-old Weanling Rats
Intestinal Adaptation Following Massive Ileocecal Resection in 20-day-old Weanling Rats
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DOI：
10.1097/mpg.0b013e3181c2c2af
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发表时间：
2010-01-01
影响因子：
2.9
通讯作者：
Kock, Nancy D.
中科院分区：
文献类型：
--
作者：
Yang, Qing;Kock, Nancy D.
目的：目前研究婴儿短肠综合征（SBS）的动物模型较少。大多数SBS模型涉及切除近端小肠，然后进行空回肠吻合术，这与人类婴儿SBS的临床相关性尚不清楚，后者通常由坏死性小肠结肠炎的手术治疗引起，并涉及切除回肠、回盲瓣和部分或整个结肠。我们的目标是开发一个更合适的SBS模型在发展中rates.Materials和方法：20日龄断奶大鼠分为2个手术组，回盲部切除术（ICR）和假手术组，对照组，不进行手术。术后7天或对照组7天自由进食流质饮食，每天记录体重、摄食量和粪便变化。然后对大鼠实施安乐死，并记录肠长度和重量。从远端空肠和近端结肠收集肠样品进行组织学检查。从空肠中段、远端和结肠采集粘液样品，用于测量粘膜重量、DNA、RNA和蛋白质水平。麦芽糖酶活性测定在小肠。结果：百分之八十五的大鼠存活ICR随后发展腹泻，食欲过旺，和不良的增长。与假手术相比，ICR的适应性反应，证明肠和粘膜重量，DNA，RNA和蛋白质水平，增加麦芽糖酶活性和绒毛厚度在远端空肠，并增加粘膜厚度在colon.Conclusions：这个ICR模型在断奶大鼠是合适的研究人类婴儿SBS。
Objective: Few infant animal models have been used to study infantile short bowel syndrome (SBS). Most SBS models involve removal of the proximal small bowel followed by jejunoileal anastomosis, which has unclear clinical relevance to human infantile SBS that often results from Surgical treatment for necrotizing enterocolitis and involves removal of the ileum, ileocccal valve, and part of or the entire colon. Our objective was to develop a more appropriate SBS model in developing rats.Materials and Methods: Twenty-day-old weanling rats were divided into 2 surgery groups, ileocecal resection (ICR) and sham groups, and a control group that did not undergo surgery. All were fed a liquid diet ad libitum for 7 days after surgery or for 7 days in the controls, and body weight, food intake, and stool changes were recorded daily. The rats were then euthanized and intestinal lengths and weights, were recorded. Samples of intestine from the distal jejunum and proximal colon were collected for histology. Mucosal samples from the middle, distal jejunum, and colon were collected for measurements of mucosal weights, DNA, RNA, and protein levels. Maltase activity was determined in the small intestine.Results: Eighty-five percent of rats survived the ICR with subsequent development of diarrhea, hyperphagia, and poor growth. Adaptive responses to ICR, as compared with sham, were evidenced by increased intestinal and mucosal weights, DNA, RNA, and protein levels, increased maltase activity and villous thickness in distal jejunum, and increased mucosal thickness in the colon.Conclusions: This ICR model in weanling rats is appropriate for studying human infantile SBS.