Identification of Lactobacillus species using tDNA-PCR
Identification of Lactobacillus species using tDNA-PCR
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DOI：
10.1016/s0167-7012(02)00035-0
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发表时间：
2002-08-01
影响因子：
2.2
通讯作者：
Haesebrouck, F
中科院分区：
文献类型：
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作者：
Baele, M;Vaneechoutte, M;Haesebrouck, F
tDNA基因间间隔物PCR （tDNA-PCR）利用tRNA基因保守边缘互补的一致引物扩增基因间间隔物。用毛细管电泳分离PCR产物，可以区分只有一个碱基对长度不同的片段。将该方法应用于37种82株乳酸菌，以评价该方法在该属内的鉴别能力。基于独特的tDNA指纹图谱，可将21种植物区分到种级。其他种类分为两个（如发酵乳杆菌和纤维乳杆菌）或三个（嗜酸乳杆菌、鸡乳杆菌和helveticus乳杆菌）。将所得指纹纳入包含许多其他革兰氏阳性和革兰氏阴性物种指纹的数字数据库，使鉴定未知菌株成为可能。(C) 2002 Elsevier Science B.V.版权所有
tDNA intergenic spacer PCR (tDNA-PCR) using consensus primers complementary to the conserved edges of the tRNA genes can amplify the intergenic spacers. Separation of the PCR products with capillary electrophoresis enables discrimination between fragments differing only one basepair in length. This method was applied to a collection of 82 Lactobacillus strains belonging to 37 species in order to evaluate the discriminatory power of this technique within this genus. Twenty-one species could be distinguished to species level on the basis of a unique tDNA fingerprint pattern. The other species grouped by two (e.g. L. fermentum and L. cellobiosus) or three (L. acidophilus, L. gallinarum and L. helveticus). Inclusion of the resulting fingerprints in a numerical database containing fingerprints of numerous other Gram-positive and Gram-negative species makes the identification of unknown strains possible. (C) 2002 Elsevier Science B.V. All rights reserved.