Cyclic estradiol treatment normalizes body weight and test meal size in ovariectomized rats
Cyclic estradiol treatment normalizes body weight and test meal size in ovariectomized rats
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DOI：
10.1016/s0031-9384(99)00060-8
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发表时间：
1999-08-01
影响因子：
2.9
通讯作者：
Asarian, L
中科院分区：
文献类型：
--
作者：
Geary, N;Asarian, L
我们测试了周期雌二醇治疗，就像持续雌二醇治疗一样，是否足以使去卵巢大鼠的膳食大小和体重正常化。实验一：成年Long-Evans大鼠摘除卵巢，每周二、三分别皮下注射0、0.2、2.0 mU的芝麻油苯甲酸雌二醇(EB)。在自由喂养的4周内，去卵巢大鼠的体重(48+/-5g)高于正常大鼠(16+/-1g，p<；0.01)。用2.0mgEB循环处理后，血浆雌二醇水平出现了与正常循环大鼠相似的变化：第二次注射EB后，雌二醇水平达到190+/-60pmol/L，到周期结束时降至检测不到的水平(>；30pmo1/L)。在实验2中，测量了过夜食物剥夺后的试验膳食大小。2.0mgEB的周期处理既产生补充性(即在周期开始时，雌二醇组大鼠的进食量比油组小)，又产生阶段性(即EB处理周期后期比早期小)的进食量减少。因此，每周周期的雌二醇治疗方案产生与正常循环大鼠相似的血浆雌二醇浓度变化，足以产生具有正常下丘脑-垂体-性腺功能特征的体重和膳食大小模式。(C)1999年爱思唯尔公司。
We tested whether cyclic estradiol treatment, like continuous estradiol treatment, is sufficient to normalize meal size and body weight in ovariectomized rats. In Experiment 1, adult Long-Evans rats were ovariectomized and subcutaneously injected with 0, 0.2, or 2.0 mu g estradiol benzoate (EB) in sesame oil each Tuesday and Wednesday. Oil-treated ovariectomized rats gained more weight during 4 weeks of ad lib feeding (48 +/- 5 g) than intact rats (16 +/- 1 g, p < 0.01). Cyclic treatment with 2.0 mu g EB produced excursions of plasma estradiol that appeared similar to those of intact, cycling rats: estradiol level reached 190 +/- 60 pmol/L after the second EB injection before decreasing to undetectable levels (> 30 pmol/L) by cycle end. In Experiment 2, test meal sizes after overnight food deprivation were measured. Cyclic treatment with 2.0 mu g EB produced both tonic (i.e., at cycle onset, meal size was smaller in estradiol-treated than oil-treated rats) and phasic (i.e., meal size was smaller late in the EB-treatment cycle than early in it) decreases in meal size. Thus,a weekly cyclic regimen of estradiol treatment that produces changes in plasma estradiol concentration similar to those in intact cycling rats is sufficient to product the body weight and meal size patterns that characterize normal hypothalamic-pituitary-gonadal function. (C) 1999 Elsevier Inc.