Tributyltin Alters Osteocalcin, Matrix Metalloproteinase 20 and Dentin Sialophosphoprotein Gene Expression in Mineralizing Mouse Embryonic Tooth in vitro
Tributyltin Alters Osteocalcin, Matrix Metalloproteinase 20 and Dentin Sialophosphoprotein Gene Expression in Mineralizing Mouse Embryonic Tooth in vitro
复制标题
三丁基锡改变体外矿化小鼠胚牙中的骨钙素、基质金属蛋白酶 20 和牙本质唾液酸磷蛋白基因表达
DOI：
10.1159/000327529
复制
发表时间：
2011
影响因子：
2.7
通讯作者：
P. Lukinmaa
中科院分区：
文献类型：
--
作者：
E. Salmela;S. Alaluusua;C. Sahlberg;P. Lukinmaa
我们在先前的体外研究中发现，三丁基锡（TBT）在发育中的小鼠牙齿中阻止牙本质矿化和釉质形成。本研究旨在探讨TBT对牙体硬组织矿化相关基因表达的影响。采用实时定量聚合酶链反应（RT-QPCR）技术，检测18天胚胎鼠下颌第一磨牙在1.0 μM TBT作用下，骨钙素（Ocn）、碱性磷酸酶（Alpl）、牙本质基质蛋白1（Dmp 1）、牙本质涎磷蛋白（Dspp）和基质金属蛋白酶20（Mmp-20）的表达。RT-QPCR显示表达有变化的组织切片进行原位杂交分析。原位杂交显示TBT降低成牙本质细胞OCN的表达，但增加上皮牙室的表达。在QPCR测定中，整个牙齿中的净效应是表达增加。TBT还降低成釉细胞和成牙本质细胞中Mmp-20的表达。Dspp表达各不相同，但QPCR检测和原位杂交均显示出下降趋势。TBT暴露对Alpl和Dmp 1表达无明显影响。成釉器上皮Ocn表达增加可能抑制牙本质矿化和釉质形成。成牙本质细胞中Ocn、Mmp-20和Dspp表达的降低可能表明细胞分化延迟，或者TBT可能特异性地降低参与牙本质矿化的基因的表达。TBT降低成釉细胞中Mmp-20的表达可能会损害釉质矿化，同时降低成牙本质细胞中Mmp-20和Dspp的表达可能会延迟牙本质矿化。
We showed in a previous in vitro study that tributyltin (TBT) arrests dentin mineralization and enamel formation in developing mouse tooth. The present aim was to investigate the effect of TBT on the expression of genes associated with mineralization of dental hard tissues. Embryonic day 18 mouse mandibular first molars were cultured for 3, 5 or 7 days and exposed to 1.0 µM TBT and studied by real-time quantitative polymerase chain reaction (RT-QPCR) for the expressions of osteocalcin (Ocn), alkaline phosphatase (Alpl), dentin matrix protein 1 (Dmp1), dentin sialophosphoprotein (Dspp) and matrix metalloproteinase 20 (Mmp-20).Ocn, Mmp-20 and Dspp, whose expressions showed changes in RT- QPCR, were further analyzed by in situ hybridization of tissue sections. In situ hybridization showed that TBT decreased Ocn expression in odontoblasts but increased the expression in the epithelial tooth compartment. In QPCR assays, the net effect in the whole tooth was increased expression. TBT also reduced Mmp-20 expression in ameloblasts and odontoblasts. Dspp expression varied but both QPCR assays and in situ hybridization showed a decreasing trend. TBT exposure had no clear effect on Alpl and Dmp1 expressions. Increased Ocn expression by epithelial enamel organ may inhibit dentin mineralization and enamel formation. Decreased Ocn, Mmp-20 and Dspp expressions in odontoblasts may indicate delayed cell differentiation, or TBT may specifically decrease the expression of genes involved in dentin mineralization. While decreased Mmp-20 expression by TBT in ameloblasts may impair enamel mineralization, the coincident reduction in Mmp-20 and Dspp expressions in odontoblasts may potentiate the delay of dentin mineralization.
影响因子：
6
作者：
Harmey, D;Hessle, L;Millán, JL
通讯作者：
Millán, JL
影响因子：
2.9
作者：
ROMBERG, RW;WERNESS, PG;MANN, KG
通讯作者：
MANN, KG