Systematic transcriptomic and metabolomic analysis of walnut (Juglans regia L.) fruit to trace variations in antioxidant activity during ripening
Systematic transcriptomic and metabolomic analysis of walnut (Juglans regia L.) fruit to trace variations in antioxidant activity during ripening
复制标题
DOI：
10.1016/j.scienta.2021.110849
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Yunjiang Cheng
中科院分区：
文献类型：
--
作者：
Ping Wang;Linlin Zhong;Hongbing Yang;Xujie Hou;Cuiyun Wu;Rui Zhang;Jun YU;Yunjiang Cheng
Walnut is considered as a good source of antioxidants. However, the molecular mechanism for the composition and accumulation of antioxidant-related metabolites in walnuts remains elusive. In this study, a comprehensive transcriptomic and metabolomic analysis was carried to reveal the metabolic dynamics, antioxidant activity variations and the underlying regulatory networks during walnut development. The results demonstrated that walnut development goes through the stages of cell division and proliferation, nutrient accumulation, and nutrient stabilization and maintenance, and the antioxidant activity first decreases and then increases. Metabolomic analysis identified a total of 176 metabolites in walnuts. Hydrolyzed tannins (HT), coumarins and their derivatives (COA) are significantly positively correlated with the antioxidant activity. Phenylpropane metabolism is the key pathway for HT and COA synthesis and the key regulatory enzymes are CHI, CHS, F3H and UGT. Sugars and organic acids provide energy and precursor for the accumulation of nutrients in walnuts through sugar metabolism and the tricarboxylic acid cycle (TCA). Large amounts of amino acids are accumulated at the nutrient accumulation stage, providing precursors for the formation of proteins and flavonoids during the ripening of walnuts. Our results provide new insights into the metabolic regulation of antioxidant activity during walnut development and ripening.
