Transformation of Arabidopsis with a Brassica SLG/SRK region and ARC1 gene is not sufficient to transfer the self-incompatibility phenotype
Transformation of Arabidopsis with a Brassica SLG/SRK region and ARC1 gene is not sufficient to transfer the self-incompatibility phenotype
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DOI：
10.1007/s004380051213
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发表时间：
2000-05-01
期刊：
影响因子：
--
通讯作者：
Rothstein, SJ
中科院分区：
文献类型：
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作者：
Bi, YM;Brugière, N;Rothstein, SJ
自交不亲和性（SI）促进开花植物的远缘杂交，并且在芸苔属中SI由S基因座遗传控制。自交不亲和的芸苔属和自交可育的拟南芥属同属十字花科。此外，对比分析表明，B之间的微共线性程度较高。油菜S基因座及其在拟南芥中的同源区域-值得注意的例外是缺少芸苔属Sl基因SLG和SRK。芸苔属ARCI编码SRK信号转导途径的一个组分，导致自交花粉排斥，在拟南芥中没有发现与之密切相关的ARC 1同源物。本研究的目的是将芸苔属植物SI组分导入拟南芥，以弥补缺失的基因，并研究SI表型是否可以转移。来自跨越B的fosmid克隆F20和F22的约40 kb的DNA片段。将甘蓝型油菜W1 SLG-SRK基因克隆到植物转化载体pBIBAC 2中。产生在花蕾中表达芸苔属SI基因的转基因植物。此外，内源性SLG样基因AtS 1没有被芸苔属SLG转基因共抑制。在T1 BIBAC 2-F20和BIBAC 2-F22转基因植物中未观察到SI表型。当ARCI基因转化到BIBAC 2-F20或BIBAC 2-F22植物中时。所得到的BIBAC 2-F20-ARC 1和BIBAC 2-F22-ARC 1植株仍然正常结实，并且当自交不亲和B时没有观察到排斥反应。将欧洲油菜W1花粉置于BIBAC 2-F20-ARC 1或BIBAC 2-F22-ARC 1拟南芥柱头上。综上所述，我们的研究结果表明，拟南芥基因组与芸苔属SLC，SRK和ARC 1基因的互补是不太可能足以转移SI表型。
Self-incompatibility (SI) promotes outbreeding in flowering plants, and in Brassica SI is genetically controlled by the S locus. Self-incompatible Brassica and self-fertile Arabidopsis belong to the same crucifer family. In addition, a comparative analysis reveals a high degree of microsynteny between the B. campestris S locus and its homologous region in Arabidopsis - with the notable exception that the Brassica SI genes, SLG and SRK, are missing. Brassica ARCI encodes a component of the SRK signal transduction pathway leading to self-pollen rejection, and no closely related ARC1 homolog has been identified in Arabidopsis. The purpose of the research reported here was to introduce Brassica SI components into Arabidopsis in an attempt to compensate for the missing genes and to investigate whether the SI phenotype can be transferred. Inserts of approximately 40 kb from the fosmid clones F20 and F22, which span the B. napus W1 SLG-SRK region, were cloned into the plant transformation vector pBIBAC2. Transgenic plants were generated that expressed the Brassica SI genes in the flower buds. In addition, the endogenous, SLG-like, gene AtS1 was not co-suppressed by the Brassica SLG transgene. No SI phenotype was observed among the T1 BIBAC2-F20 and BIBAC2-F22 transgenic plants. When the ARCI gene was transformed into BIBAC2-F20 or BIBAC2-F22 plants. the resulting BIBAC2-F20-ARC1 and BIBAC2-F22-ARC1 plants still set seeds normally, and no rejection response was observed when self-incompatible B. napus W1 pollen was placed on BIBAC2-F20-ARC1 or BIBAC2-F22-ARC1 Arabidopsis stigmas. Taken together, our results suggest that complementing Arabidopsis genome with Brassica SLC, SRK and ARC1 genes is unlikely to be sufficient to transfer the SI phenotype.