Hill Reaction, Hydrogen Peroxide Scavenging, and Ascorbate Peroxidase Activity of Mesophyll and Bundle Sheath Chloroplasts of NADP-Malic Enzyme Type C(4) Species.
Hill Reaction, Hydrogen Peroxide Scavenging, and Ascorbate Peroxidase Activity of Mesophyll and Bundle Sheath Chloroplasts of NADP-Malic Enzyme Type C(4) Species.
复制标题
NADP-苹果酸酶 C(4) 型叶肉和束鞘叶绿体的希尔反应、过氧化氢清除和抗坏血酸过氧化物酶活性。
DOI：
10.1104/pp.85.1.294
复制
发表时间：
1987
期刊：
影响因子：
7.4
通讯作者：
G. Edwards
中科院分区：
文献类型：
--
作者：
Y. Nakano;G. Edwards
使用酶消化和机械隔离技术从NADP-麦芽酶C（4）植物玉米，高粱（单子叶植物）和Flaveria trinervia（Dicot）中分离出的完整的叶叶叶叶绿体叶绿体叶绿体。该C（4）亚组的束护套叶绿体往往是Agranal的，以前据报道缺乏光系统II活性。然而，在将完整的束鞘叶氯植物注射到低音培养基中后，类囊体具有高的山山反应活性，类似于与山叶氯植物成形棒类似的山丘氧化剂二氯苯酚，P-二甲醇，二苯甲酰酮和铁苯胺（大约200至300微米o（2）微米o（2）evoloved per per， mg叶绿素每小时）。与叶绿素叶绿体相比，玉米和高粱的捆鞘叶绿体的丘陵反应活性在测定过程中不稳定，活性却失去了活性。玉米的束鞘叶绿色叶绿体还表现出某种能力的3-磷酸甘油酸含量O（2）进化（29至58个微孔O（2）每小时每毫克叶绿素进化）。在光过氧化氢的光清除中，叶肉和捆鞘叶绿体同样有效。结果表明，两种叶绿体类型均具有非环状电子转运和酶学，以减少过氧化氢的水。这些叶绿体类型的抗坏血酸过氧化物酶的活性与它们清除过氧化氢的能力一致。
Intact mesophyll and bundle sheath chloroplasts wee isolated from the NADP-malic enzyme type C(4) plants maize, sorghum (monocots), and Flaveria trinervia (dicot) using enzymic digestion and mechanical isolation techniques. Bundle sheath chloroplasts of this C(4) subgroup tend to be agranal and were previously reported to be deficient in photosystem II activity. However, following injection of intact bundle sheath chloroplasts into hypotonic medium, thylakoids had high Hill reaction activity, similar to that of mesophyll chloroplasts with the Hill oxidants dichlorophenolindophenol, p-benzoquinone, and ferricyanide (approximately 200 to 300 micromoles O(2) evolved per mg chlorophyll per hour). In comparison to that of mesophyll chloroplasts, the Hill reaction activity of bundle sheath chloroplasts of maize and sorghum was labile and lost activity during assay. Bundle sheath chloroplasts of maize also exhibited some capacity for 3-phosphoglycerate dependent O(2) evolution (29 to 58 micromoles O(2) evolved per milligram chlorophyll per hour). Both the mesophyll and bundle sheath chloroplasts were equally effective in light dependent scavenging of hydrogen peroxide. The results suggest that both chloroplast types have noncyclic electron transport and the enzymology to reduce hydrogen peroxide to water. The activities of ascorbate peroxidase from these chloroplast types was consistent with their capacity to scavenge hydrogen peroxide.